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2 cationic polyacrylamide gel electrophoresis in colombia

  • Two Dimensional Anionic-Cationic Detergents

    1985-1-1 · Fig.2 (right) Polyacrylamide gel electrophoresis in the presence of TDAB. Lane 1, 2, 3 as in Fig. 1. The separating gel (15% acrylamide) and the stacking gel (4% acrylamide) were prepared as described by Amory et al, (1980), except that sodium phosphate buffer replaced potassium phosphate.

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  • Polyacrylamide Gel Electrophoresis | Cleaver

    An improved system for polyacrylamide gel electrophoresis in the presence of cationic detergents, cetyltrimethylammonium bromide and cetylpyridinium chloride, respectively, is described. An acidic discontinuous buffer system generated according to the theory of multiphasic zone electrophoresis developed by T. M. Jovin (1973, Biochemistry 12 ...

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  • Use of cationic detergents for polyacrylamide

    Cationic ionic liquids-based surfactants (ILS), such as 4-methyl pyridinium bromide (C n PBr, where n=4,6,8), were used in preparation of polyacrylamide gels, sample buffer, and running buffer for cationic ILS polyacrylamide gel electrophoresis (ILS-PAGE). These ILS are liquids in the pure state and were selected for improved separation of ribonuclease …

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  • Cationic ionic liquid surfactant-polyacrylamide

    Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over a wide …

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  • [PDF] Polyacrylamide Gel Electrophoresis |

    Polyacrylamide (12.5 %) gel slab electrophoresis in 0.1% sodium dodecyl sulphate was carried out as described by Laemmli (9). Analytical IEF in the pH range 2.5–7.0 was performed on 5.0 % acrylamide gel slab using a Multiphor II electrophoresis system (Pharmacia Biotech) following the manufacturer's instructions. Non-denaturing.

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  • Polyacrylamide - an overview | ScienceDirect

    1. Anal Biochem. 1975 Feb;63(2):345-9. Influence of a cationic detergent on electrophoresis in polyacrylamide gel. Schick M. PMID: 235854 [Indexed for MEDLINE]

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  • Influence of a cationic detergent on

    1984-8-22 · 1. Biochim Biophys Acta. 1984 Aug 22;775(2):239-45. Two-dimensional gel electrophoresis of membrane proteins using anionic and cationic detergents. Application to the study of mitochondrial F0-F1-ATPase. Penin F, Godinot C, Gautheron DC.

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  • Two-dimensional gel electrophoresis of

    Cationic electrophoresis. Buxbaum E(1). Author information: (1)Ross University School of Medicine, Roseau, Commonwealth of Dominica, West Indies. [email protected] Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist.

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  • Cationic electrophoresis.

    Freshly collected parotid saliva collected from human donors were shown by polyacrylamide gel electrophoresis to continuously secrete a group of low-molecular-weight cationic polypeptides. Up to 14 bands could be identified by Coomassie blue staining, and all bands migrated more rapidly than purified human leukemic lysozyme in cationic …

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  • Cationic Electrophoresis | SpringerLink

    2012-4-9 · Eley M, Burns P, Kannapell C et al (1979) Cetyltrimethylammonium bromide polyacrylamide gel electrophoresis: estimation of protein subunit molecular weights using cationic detergents. Anal Biochem 92:411–419 PubMed CrossRef Google Scholar

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  • Gel Electrophoresis of Proteins - GBV

    2013-3-1 · 2. Fundamentals of capillary gel electrophoresis 106 3. Operational variables 108 Applied electric field and temperature 109 Capillary dimensions 109 Capillary coating 110 Gels and polymer solutions 112 4. Separations 113 Cross-linked polyacrylamide gel 113 Linear polyacrylamide solutions 115 Other entangled polymer solutions 116 The …

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  • Global Polyacrylamide Gel Electrophoresis

    2021-7-7 · Polyacrylamide gel electrophoresis (PAGE) is a typical technique for protein separation by electrophoresis.In this technique, polyacrylamide gel is used as the base medium, while sodium dodecyl sulfate (SDS) is used for protein chain and protein denaturation. Market Analysis and Insights: Global ...

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  • [PDF] Polyacrylamide Gel Electrophoresis |

    Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over a wide …

    Get Price
  • Microfluidic Polyacrylamide Gel Electrophoresis

    2009-9-4 · Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting. Integration of three PA gel elements into a glass microfluidic chip achieved multiple functions, including (1) rapid protein separation via on-chip PAGE, (2) directed electrophoretic transfer of resolved protein peaks to an in-line …

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  • polyacrylamide, polyacrylamide Suppliers and

    Polyacrylamide Polyacrylamide Cationic Polyacrylamide Flocculating Agent. 2.80 Kilogram. 1000 Kilograms (Min. Order) Xinxiang Kolanky Technical Co., Ltd. CN 8 YRS. Contact Supplier. Ad. 1/4.

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  • Label-free horizontal EMSA for analysis of protein

    Non-denaturing gel electrophoresis . Separating gels were 5% polyacrylamide (diluted from a 40% stock of acrylamide:bis-acrylamide, 38:2) prepared in a buffer composed of 30 mM MOPS/25 mM histidine, pH 6.5. To speed polymerization, the gel mix (50 mL) was heated in a microwave oven for 30 sec, after

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  • PURIFICATION AND PARTIAL CHARACTERIZATION

    2020-1-19 · Chiriguaná, Colombia (9°22´N; 73°37´W). Five fractions were obtained by the gel filtration, and PLA2 activity was found in fraction 3 (F3). In the cationic exchange chromatography, F3 showed eight components with PLA2 activity. Six of these components appeared as one band in polyacrylamide gel electrophoresis (SDS-PAGE).

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  • Microfluidic Polyacrylamide Gel Electrophoresis

    2009-9-4 · Native polyacrylamide gel electrophoresis (PAGE) was integrated with subsequent in situ immunoblotting. Integration of three PA gel elements into a glass microfluidic chip achieved multiple functions, including (1) rapid protein separation via on-chip PAGE, (2) directed electrophoretic transfer of resolved protein peaks to an in-line …

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  • In silico-screened cationic dipeptides from

    2021-4-24 · In this paper, some cationic dipeptides from scallop (Patinopecten yessoensis) male gonads (SMGs), which can synergistically gel with ι-carrageenan (ι-C), were screened by the in silico approach.Fourteen protein sequences of SMGs were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) …

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  • Heterogeneity of Bovine Fibrinogen and Fibrin

    2020-11-5 · Polyacrylamide Gel Electrophoresis-Polyacrylamide gel elec- trophoresis in 0.1% sodium dodecyl sulfate at pH 7.0 was per- formed in 5% gels using the method of Weber and Osborn (21). Prior to electrophoresis, samples were incubated for 10 min at

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  • Simple visualization of protein bands in SDS ...

    Simple visualization of protein bands in SDS-polyacrylamide gel electrophoresis by the insoluble complex formation between SDS and a cationic surfactant. Takagi T, Kubo K, Isemura T. Analytical Biochemistry, 01 May 1977, 79(1-2): 104-109 DOI: 10.1016/0003 ...

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  • Electrophoretic Techniques - Proteomics in

    2008-3-26 · Polyacrylamide Gel Electrophoresis The Polyacrylamide Gel. SDS Polyacrylamide Gel Electrophoresis. Blue Native Electrophoresis. Cationic Detergent Electrophoresis. Blotting Electrophoretic Transfer. Protein Detection on the Membrane. Isoelectric Focusing Theoretical Background. Preparation of IEF Gels

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  • Label-free horizontal EMSA for analysis of protein

    Non-denaturing gel electrophoresis . Separating gels were 5% polyacrylamide (diluted from a 40% stock of acrylamide:bis-acrylamide, 38:2) prepared in a buffer composed of 30 mM MOPS/25 mM histidine, pH 6.5. To speed polymerization, the gel mix (50 mL) was heated in a microwave oven for 30 sec, after

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  • Multiphasic zone electrophoresis. II. Design of

    2019-5-25 · Biochemistry All Publications/Website. OR SEARCH CITATIONS

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  • Criterion™ Tris-HCl Precast Gels | Life Science

    Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells This product is being discontinued soon. Please contact customer service for more information. 15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

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  • Influence of a cationic detergent on

    Use of cationic detergents for polyacrylamide gel electrophoresis in multiphasic buffer systems. Mócz G , Bálint M Anal Biochem , 143(2):283-292, 01 Dec 1984

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  • Simple visualization of protein bands in SDS ...

    Simple visualization of protein bands in SDS-polyacrylamide gel electrophoresis by the insoluble complex formation between SDS and a cationic surfactant. Takagi T, Kubo K, Isemura T. Analytical Biochemistry, 01 May 1977, 79(1-2): 104-109 DOI: 10.1016/0003 ...

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  • On-line microchip electrophoresis-mediated ...

    Poly(NIPAM-co-MAA-co-ODA), functionalized polyNIPAM, functionalized polyacrylamide, Polyacrylamide, polyNIPAM Linear Formula : (C 6 H 11 NO) m (C 4 H 6 O 2 ) n (C 21 H 40 O 2 …

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  • Polyacrylamide | Sigma-Aldrich

    2018-10-2 · Abstract. The substitution of the reverse polarity benzyldimethyl-n-hexadecylammonium chloride (16-BAC) polyacrylamide gel electrophoresis (PAGE) for isoelectric focusing (IEF) in the first dimension of electrophoresis improves the solubility of extremely hydrophobic proteins and their recovery compared to conventional 2D …

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  • Two-Dimensional 16-BAC/SDS Polyacrylamide

    2020-1-19 · Chiriguaná, Colombia (9°22´N; 73°37´W). Five fractions were obtained by the gel filtration, and PLA2 activity was found in fraction 3 (F3). In the cationic exchange chromatography, F3 showed eight components with PLA2 activity. Six of these components appeared as one band in polyacrylamide gel electrophoresis (SDS-PAGE).

    Get Price
  • PURIFICATION AND PARTIAL CHARACTERIZATION

    gel-based electrophoresis techniques, SDS-PAGE14–17 and 2-DE, 15,16,18 Blue-native 19,20 and Native-PAGE 21,22 are important and the most commonly used methods for protein separation.

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  • Ionic liquids as buffer additives in ionic liquid ...

    The system combines discontinuous gel electrophoresis in an arginine/N-Tris (hydroxymethyl) methylglycine) (Tricine) buffer with sample solubilization in cetyltrimethylammonium bromide (CTAB). Because the components that distinguish this system are CTAB, arginine, and Tricine and because CTAB is a cationic detergent, we …

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  • Cetyltrimethylammonium Bromide Discontinuous

    Solid support media used in zonal electrophoresis (3) Cellulose acetate, agarose gel, PAGE (polyacrylamide gel) Most common solid support media used in electrophoresis

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  • Two-Dimensional Polyacrylamide Gel

    2019-7-22 · In an effort to develop an analytical method capable of finding new metalloproteins, this is the first report of a new diagonal gel electrophoresis method to isolate and identify metalloproteins ...

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  • Polyacrylamide Gel Identification of Bacterial

    Polyacrylamide Gel Identification of Bacterial L-Forms andMycoplasma Species of HumanOrigin ... cells on cationic polyacrylamide gels has been used successfully for the identification ofvarious Mycoplasma serotypes (12, 15, 18, 19, 20) and ... Atpresent, gel electrophoresis is the onlyprac-tical method for the identification of bacterial L-forms.

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  • Enzyme Purification by Electrophoresis - Creative

    2014-4-15 · Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to simultaneously fractionate, identify, and quantify proteins when coupled with …

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  • Basics and recent advances of two dimensional ...

    Polyacrylamide gel electrophoresis: Electrophoresis for cationic proteins was performed in 10% polyacrylarnide gels (19). SDS-polyacrylamide gel electrophoresis and autoradiography were carried out as described elsewhere (14). Phagocytosis: The effect of phagocytosis on the production and secretion of lysozyme was

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  • IN VITRO SYNTHESIS AND SECRETION OF

    In-gel staining of proteins in native polyacrylamide gel electrophoresis using meso-tetrakis(4-sulfonatophenyl) porphyrin K. Divakar, G. Nandhini Devi, and Pennathur Gautam Rapid detection of proteins in polyacrylamide electrophoresis gels with direct red 81 and amido black David Choveaux, Robert G.E. Krause, and J.P. Dean Goldring

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  • Protein electrophoresis : methods and protocols

    2014-4-5 · Conventional polyacrylamide gel electrophoresis (7.5) 10 In this study, anodic conventional polyacrylamide gel electrophoresis (7.5%) was carried out according to LKB 2117 note using tris-glycine buffer stock solution (0.15 M) pH 8.9, for separation of peroxidase. Cathodic polyacrylamide gels electrophoresis (9%)

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  • Electrophoresis Profile of Total Peroxidases in Sera

    multiSUB Choice, Wide Midi Horizontal Electrophoresis System. Three tray options. SKU: See Product Page. £ 285.00 – £ 339.00 ex. VAT. Find out more.

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  • Buy Electrophoresis Products Online | Cleaver

    US8053213B2 US12/876,320 US87632010A US8053213B2 US 8053213 B2 US8053213 B2 US 8053213B2 US 87632010 A US87632010 A US 87632010A US 8053213 B2 US8053213 B2 US 8053213B2 Authority US United States Prior art keywords dna binding sample pcr gel nucleic acid Prior art date 2008-04-08 Legal status (The legal status is …

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  • US8053213B2 - Detection of PCR products in gel ...

    2021-5-15 · Die BAC-PAGE eignet sich zur Trennung von Proteinen mit vielen positiven Ladungen oder glykosylierten Membranproteinen und zur 2D-Gelelektrophorese von Membranproteinen eingesetzt, darunter auch zur Trennung mitochondrialer Membranproteine. Im Gegensatz zu üblicherweise verwendeten SDS-PAGE wandern die …

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  • Two-Dimensional Polyacrylamide Gel

    2019-7-22 · In an effort to develop an analytical method capable of finding new metalloproteins, this is the first report of a new diagonal gel electrophoresis method to isolate and identify metalloproteins ...

    Get Price
  • 5.5: Gel Electrophoresis of Proteins - Biology

    2021-3-5 · The gel matrix for gel electrophoresis of proteins. Gel electrophoresis of proteins almost exclusively utilizes polyacrylamide. This is a polymer comprised of two covalently-linked components: acrylamide; bis acrylamide. The bis acrylamide is essentially a cross-linking component of the acrylamide polymer.

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  • Basics and recent advances of two

    2014-4-15 · Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to simultaneously fractionate, identify, and quantify proteins when coupled with …

    Get Price
  • In Situ Fabrication of Ionic Polyacrylamide

    2008-10-8 · Microchip electrophoresis utilizing an in situ photopolymerized Phos-tag binding polyacrylamide gel for specific entrapment and analysis of phosphorylated compounds. The Analyst 2017, 142 (18) , 3416-3423. DOI: 10.1039/C7AN00836H.

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  • One‐Dimensional Electrophoresis Using

    2014-4-5 · Conventional polyacrylamide gel electrophoresis (7.5) 10 In this study, anodic conventional polyacrylamide gel electrophoresis (7.5%) was carried out according to LKB 2117 note using tris-glycine buffer stock solution (0.15 M) pH 8.9, for separation of peroxidase. Cathodic polyacrylamide gels electrophoresis (9%)

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  • Electrophoresis Profile of Total Peroxidases in Sera

    Gel electrophoresis - Coggle Diagram: Gel electrophoresis. linear relationship between the logarithm of the molecular weight and the relative distance of migration of the SDS-polypeptide complex.

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  • Gel electrophoresis - Coggle Diagram

    2009-7-17 · Polyacrylamide gel electrophoresis (PAGE) is used to separate the component of the glutathione protected gold clusters up to Au160 core size and compare the size with the mobility data from PAGE. Both electrospray mass spectrometry and transmission electron microscopy were systematically applied to analyze …

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  • Polyacrylamide (PAM) For Sale - Water

    2019-5-25 · Biochemistry All Publications/Website. OR SEARCH CITATIONS

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  • Two-Dimensional Polyacrylamide Gel

    2019-7-22 · In an effort to develop an analytical method capable of finding new metalloproteins, this is the first report of a new diagonal gel electrophoresis method to isolate and identify metalloproteins ...

    Get Price
  • Basics and recent advances of two dimensional ...

    2014-4-15 · Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to simultaneously fractionate, identify, and quantify proteins when coupled with …

    Get Price
  • Label-free horizontal EMSA for analysis of protein

    Non-denaturing gel electrophoresis . Separating gels were 5% polyacrylamide (diluted from a 40% stock of acrylamide:bis-acrylamide, 38:2) prepared in a buffer composed of 30 mM MOPS/25 mM histidine, pH 6.5. To speed polymerization, the gel mix (50 mL) was heated in a microwave oven for 30 sec, after

    Get Price
  • Ionic liquids as buffer additives in ionic liquid ...

    gel-based electrophoresis techniques, SDS-PAGE14–17 and 2-DE, 15,16,18 Blue-native 19,20 and Native-PAGE 21,22 are important and the most commonly used methods for protein separation.

    Get Price
  • Blue native electrophoresis protocol | Abcam

    Blue native polyacrylamide gel electrophoresis (BN-PAGE) is performed essentially as described by Schä gger and von Jagow (1991), Analytical Biochemistry, 199, 223-31. First, solubilized samples are stained with a charged (Coomassie) dye. The intact mitochondrial complexes are then separated by electrophoresis based upon how much dye was …

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  • 2-Amino-2-methyl-1,3-propanediol ≥97.0% (GC) |

    2-Amino-2-methyl-1,3-propanediol also referred to as AMPD or Ammediol is a biological buffer. It is used in the resolving phase of the gel and stabilizes the pH as well as assists in separating the proteins with wide range of molecular sizes (10 kDa to over 220 kDa) using unusually low-concentrated gels.

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  • Criterion™ Tris-HCl Precast Gels | Life Science

    Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells This product is being discontinued soon. Please contact customer service for more information. 15% Criterion™ Tris-HCl Protein Gel, 12+2 well, 45 µl

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  • polyacrylamide | chemical compound | Britannica

    polyacrylamide, an acrylic resin that has the unique property of being soluble in water. It is employed in the treatment of industrial and municipal wastewater. Polyacrylamides are produced by the polymerization of acrylamide (C3H5NO), a compound obtained by the hydration of acrylonitrile.

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  • polyacrylamide gel protocol factories - badoc.info

    Extracts of the hybrids were then subjected to polyacrylamide gel electrophoresis and tested for tk activity .用聚丙烯酰胺凝胶电泳检测杂种提取种物的TK活力。Analytical polyacrylamide gel electrophoresis apparatus分析聚丙酰胺凝胶电泳仪 …

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