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20 polyacrylamide gel recipe in italy

  • 20 Tbe Polyacrylamide Gel Recipe | Bryont Rugs and

    2018-2-18 · A 20 Polyacrylamide Gel Electropsis Under Native Conditions Trupage Precast Gels Sds Polyacrylamide Gel Electropsis Polyacrylamide Gel 10 In ½ X Tbe Buffer And 20 Stock Solution

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  • SDS Polyacrylamide Gel Electrophoresis

    2011-7-14 · SDS Polyacrylamide Gel Electrophoresis Gel Recipes % Acrylamide 5% 7.5% 10.% 12.5% 15% 18% 4% Stacking Gel ... 50 ml 20% SDS 7.5 ml 20% SDS dH 2 O to 2 liters dH 2 O to 1.5 liters Laemmli Sample Preparation Buffer: DTT: 123.4 mg Glycerol or 50% sucrose 4 …

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  • Purificationof DNA using nondenaturing

    2016-8-9 · 2.Prepare the gel solution (see Table 2.7.1 for appropriate acrylamide concentrationsfor resolving DNA fragments of different sizes). For a nondenaturing 5% polyacrylamide gel of 20 cm x 16 cm x 1.6 mm, 60 ml of gel solution issufficient, and it can be made by mixing the following: 6ml 10x TBE buffer 10ml of 29:1 acrylamide/bisacrylamide 44ml of water

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  • polyacrylamide gel recipe, polyacrylamide gel recipe ...

    1,591 polyacrylamide gel recipe products are offered for sale by suppliers on Alibaba.com A wide variety of polyacrylamide gel recipe options are available to you, There are 2 suppliers who sells polyacrylamide gel recipe on Alibaba.com, mainly located in Asia.

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  • Tris-acetate polyacrylamide gradient gels for the ...

    Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show that this system is highly sensitive, …

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  • Polyacrylamide Gel Electrophoresis for Western Blot

    Western Blot is composed of polyacrylamide gel electrophoresis (PAGE), followed by an electrophoretic transfer onto a membrane (mostly PVDF or Nitrocellulose) and an immunostaining procedure to visualize a certain protein on the blot membrane. SDS-PAGE is a standard means for separating proteins according to their molecular weight.

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  • Denaturing Polyacrylamide Gel Electrophoresis

    2019-2-5 · polyacrylamide around gel. 19. Hold two pieces of dry 46 × 57–cm blotting paper together as one piece. Beginning at one end of gel and working slowly towards the other, lay paper on top of gel. Take care to prevent air bubbles from forming between paper and gel. 20. Peel blotting paper up; gel should come off plate with it. Gradually curl paper and

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    2011-12-21 · 8. Allow the gel to polymerize for 20 minutes. 9. After the running gel has polymerized, rinse the ethanol from the surface with D.water. Drain excess water. 10. Prepare the stacking gel. This is composed of 4% acrylamide Stacking gel (add the following recipe) Percentage 4% Total 10 ml 5 ml D.Water 3.35 ml 1.68 ml

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  • SDS PAGE and Western blot - NAU

    2018-11-14 · Abstract. Proteins can easily be separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the ...

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  • 20 Polyacrylamide Gel Recipe For Dna | Bryont Rugs

    2018-8-2 · 20 Polyacrylamide Gel Recipe For Dna. Uncategorized. 20 Polyacrylamide Gel Recipe For Dna. Bryont Rugs and Livings August 2, 2018. I am trying to make sds page gel but precast gels sds page sample buffer recipes lidstrom sds page openwetware. I Am Trying To Make Sds Page Gel But Unfortunately The Running

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    2013-11-4 · DNA Polyacrylamide Gel Electrophoresis How to pour and run a neutral polyacrylamide gel. Buffers and Solutions Acrylamide:bisacrylamide (29:1) (30% w/v) Ammonium persulfate (10% w/v) Ammonium persulfate is used as a catalyst for the copolymerization of acrylamide and bisacrylamide gels. The polymerization reaction is driven by free

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  • DNA Polyacrylamide Gel Electrophoresis

    Set the voltage upto 180 V and run for 1 hour.(Don't allow the dye front to go out of the gel). Staining the gel: After running, switch off the power supply and take out the gel plates, remove the gel. Place the gel in the staining solution for 30 minutes. Destain the gel until the bands are properly seen.

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  • Polyacrylamide Gel Electrophoresis (Procedure) :

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

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  • Running agarose and polyacrylamide gels

    Gel opening lever ( 456-0000 ), sold separately, is 100% aluminum and recyclable. Ready Gel polyacrylamide precast gels are designed to fit the Mini-PROTEAN ® Tetra cell and are ready to run. Simply lock them into the cell, load your samples, and get …

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  • Polyacrylamide Reagents and Precast Gels | Life

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • Polyacrylamide gel electrophoresis - OpenWetWare

    2014-7-15 · SDS and native polyacrylamide gel electrophoresis of proteins Supplies and Reagents Acrylamide solutions (see Table 1 and Table 2 for recipes) ... Components Gel Volume => 5 ml 10 ml 15 ml 20 ml 25 ml 30 ml 40 ml 50 ml 6% gel H 2O 2.6 5.3 7.9 10.6 13.2 15.9 21.2 26.5

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  • The principle and method of polyacrylamide gel ...

    Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desired resolution is achieved. (The electrophoresis can be followed by using pre-stained molecular weight markers). Set up the Submerged Western Transfer Cassette as described below and in Figure III-2:

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  • Polyacrylamide Gel Recipe Dna | Besto Blog

    2018-5-13 · Pdf Denaturing Urea Polyacrylamide Gel Electropsis Page Recipe for polyacrylamide gel recipe for polyacrylamide gel i am trying to make sds page gel but unfortunately the running smeared dna bands in polyacrylamide gels but not agarose gel. Whats people lookup in this blog: Polyacrylamide Gel Recipe For Dna; 20 Polyacrylamide Gel Recipe For Dna

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  • Running agarose and polyacrylamide gels

    2011-6-17 · The basics. Agarose gels can be used to resolve large fragments of DNA. Polyacrylamide gels are used to separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used (Figure 1). These gels can be run with or without a denaturant. Gels that are run without a denaturant are referred to as native gels.

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  • Polyacrylamide Reagents and Precast Gels | Life

    Gel opening lever ( 456-0000 ), sold separately, is 100% aluminum and recyclable. Ready Gel polyacrylamide precast gels are designed to fit the Mini-PROTEAN ® Tetra cell and are ready to run. Simply lock them into the cell, load your samples, and get …

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  • 12 Polyacrylamide Gel Recipe | Besto Blog

    2018-7-26 · Precast Gel Sds Page 4 20 8x10cm 12well Ab119209 Abcam Introduction to polyacrylamide gels lsr bio rad protocol for making sds recipe for polyacrylamide gel 1 solutions for preparing resolving gels tris glycine sds page. Whats people lookup in this blog: 12 Polyacrylamide Gel Recipe;

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  • Denaturing Polyacrylamide/Urea Gel Electrophoresis

    2018-8-22 · For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: 10X TBE Buffer 4 ml 20% acrylamide/bisacrylamide 10 ml UREA 19.2 g (to 8 M nal concentration) Deionized water to 40 ml 2. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of UREA

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  • A simple polyacrylamide gradient gel preparation

    1976-11-1 · A simple device for the preparation of continuous linear gradients in usual gel rods is described. It consists of two-tube-supporting disks of plastic on an axis, forming a rotating rack. Its versatile applicability allows the preparation of concentration, pH, buffer, and buffer strength gradients as well. The exact percentage of transmission ...

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  • Protocols - Western Transfer (Western Blot)

    Load the protein sample onto a 4-20% Tris-Glycine polyacrylamide gel and run until desired resolution is achieved. (The electrophoresis can be followed by using pre-stained molecular weight markers). Set up the Submerged Western Transfer Cassette as described below and in Figure III-2:

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  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • Non-denaturing PAGE - Thermo Fisher Scientific

    2018-8-21 · 1. For a nondenaturing 5% polyacrylamide gel solution of 40 ml, mix the following: 10X TBE Buffer 4 ml 20% acrylamide/bisacrylamide 10 ml Deionized water 26 ml 2. Caution: acrylamide is a neurotoxin; always wear gloves, safety glasses, and a surgical mask when working with acrylamide powder. 3. Vigorously agitate the solution for 1 min by

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  • Denaturing Urea PAGE - Small Gel

    2001-6-22 · 2. Pour gel. Add 25 µl TEMED and 50 µl 25% APS. Pour gel to ~ 0.5 cm from top. Insert clean, dry comb at an angle to prevent trapping of bubbles. Push all the way down, but don't trap any bubbles. 3. Pre-electrophorese gel. Use 1 X TBE in upper and lower reservoirs. Remove comb. Run at 20 watts for 15 minutes. Warmth plus urea denatures ...

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  • SDS-Polyacrylamide Gel Elec - iSpyBio

    2014-7-1 · SDS-Polyacrylamide Gel Electrophoresis of Proteins Joseph Sambrook and David W. Russell This protocol was adapted from 'Commonly Used Techniques in Molecular Cloning,' Appendix 8, in Molecular Cloning ,

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  • Polyacrylamide Gel Recipe Dna | Dandk Organizer

    8 Polyacrylamide Gel Recipe Dna Hrblock. READ Public Storage Brooklyn Ny 11213. Introduction To Page Sigma Aldrich. Polyacrylamide Gel Electropsis Page Amrita University You. Agarose Versus Polyacrylamide Not All Gels Are Created Equal. Polyacrylamide Gel An Overview Sciencedirect Topics.

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  • Denaturing Polyacrylamide/Urea Gels in TBE Buffer

    2020-8-12 · 1. Prepare 20 ml of a 5% polyacrylamide gel containing 7 M urea by adding: 47.5% acrylamide: 2.5% bis-acrylamide solution 2 ml 10 M urea 14 ml 10X TBE Buffer 2 ml 10% freshly prepared ammonium persulfate 0.2 ml Deionized water 1.8 ml 2. Mix and add 10 µl TEMED. Mix again and pour the gel carefully avoiding the formation of air bubbles. 3.

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  • Polyacrylamide Gel Electrophoresis of RNA

    There are two common types of gel: polyacrylamide and agarose. For most applications, denaturing acrylamide gels are most appropriate. These gels are extremely versatile and can resolve RNAs from ~600 to ≤20 nucleotides (nt). In certain circumstances, e.g., resolving different conformers of RNAs or RNA-protein complexes, native gels are ...

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  • SDS PAGE-Preparation

    2014-7-1 · Stacking gel (acrylamide 5%) is poured on top of the separating gel (after solidification) and a gel comb is inserted in the stacking gel. The acrylamide percentage in SDS PAGE gel depends on the size of the target protein in the sample. (details shown below) Acrylamide % M.W. Range 7% 50 kDa - 500 kDa 10% 20 kDa - 300 kDa 12% 10 kDa - 200 kDa

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  • Company - GEL S.p.A.

    2021-7-9 · The data controller is GEL S.p.A., located and registered in Via Enzo Ferrari no. 1, 60022, Castelfidardo (AN), Italy. Any further queries on personal data processing can be sent to [email protected] Type of personal data processed. The data controller processes the non-sensitive personal data of the type described in the paragraphs below.

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  • Native Polyacrylamide Gels | SpringerLink

    2018-11-14 · Abstract. Proteins can easily be separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the ...

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  • Tris–Acetate Polyacrylamide Gradient Gels for the ...

    2012-4-9 · Abstract. Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris–acetate buffer and 3–15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10–500 kDa.

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  • BASIC PROTOCOL: PURIFICATION OF

    2016-8-9 · For example,while a 20 % gel can be electrophoresed at 800 V with few problems, an8 % gel under the same conditions would likely generate too much heat forthe apparatus to dissipate. 13. When the oligonucleotideis sufficiently resolved, turn off the power supply and detach the platesfrom the electrophoresis tank.

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  • Acrylamide Gel Electrophoresis | Thermo Fisher

    Polyacrylamide gel electrophoresis provides very high resolution of DNA molecules 10–3,000 bp long. Under the appropriate conditions, DNA molecules differing in size by only a single base pair can be resolved (learn more: Nucleic acid electrophoresis education).We offer convenient reagents for polyacrylamide gel electrophoresis, including hassle-free precast Invitrogen Novex polyacrylamide ...

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  • Section VII: Separation of DNA in Polyacrylamide Gels

    — Separation of DNA ranging from 20 bp - 2000 bp in length — Study DNA-Protein interactions (Gel Shift Assays) Buffers for Electrophoresis To ensure adequate buffering power during vertical electrophoresis, TBE Buffer is used for polyacrylamide gel electrophoresis at a working strength of 1X. Lower

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  • Polyacrylamide Gel Electrophoresis of RNA

    There are two common types of gel: polyacrylamide and agarose. For most applications, denaturing acrylamide gels are most appropriate. These gels are extremely versatile and can resolve RNAs from ~600 to ≤20 nucleotides (nt). In certain circumstances, e.g., resolving different conformers of RNAs or RNA-protein complexes, native gels are ...

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  • Polyacrylamide Gel Electrophoresis (PAGE)

    2006-12-13 · POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE) Last Update: December 2006. PREPARE SOLUTIONS. 1. 30% Acrylamide 0.8% Bis (300 mL): Mix 90 g of Acrylamide, 2.4 g of N'N'-bis-methylene-acrylamide, and 300 mL of dH 2 O (filter through a 0.45 m filter) (Store at 4 o C in a dark bottle) 2. 10% SDS (100 mL): Weigh 10 g of SDS and add dH 2 O to 100 mL.

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  • Blue Native Polyacrylamide Gel Electrophoresis

    2020-4-6 · Basic Protocol: . Oligomerization of proteins controls numerous biochemical features, such as the stability of proteins and the activity of enzymes, immune receptors, and ion channels (Gell, Grant, & Mackay, 2012).Gel filtration and blue native polyacrylamide gel electrophoresis (BN-PAGE) are the two principle approaches to studying native protein oligomerization in vitro and in vivo (Fiala ...

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  • Denaturing Polyacrylamide/Urea Gels in TBE Buffer

    2020-8-12 · 1. Prepare 20 ml of a 5% polyacrylamide gel containing 7 M urea by adding: 47.5% acrylamide: 2.5% bis-acrylamide solution 2 ml 10 M urea 14 ml 10X TBE Buffer 2 ml 10% freshly prepared ammonium persulfate 0.2 ml Deionized water 1.8 ml 2. Mix and add 10 µl TEMED. Mix again and pour the gel carefully avoiding the formation of air bubbles. 3.

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  • Protocol - Tris-Tricine Peptide Separation Gels ...

    2014-4-20 · Stock Solution 29:1 acrylamide/bisacrylamide Tris-Cl/SDS, pH8.45 H2O Glycerol 10%(w/v) ammonium persulfate TEMED: Separating Gel 21.72 ml 20.00 ml 11.94 ml

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  • In-gel digestion for mass spectrometric

    2007-1-25 · In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et …

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  • Sds gel calculator - FAITID

    2019-7-31 · Allow 20-30min for a complete gelation. 2. You can prepare the stacking gel solution while the separating gel is gelating. Prepare appropriate amount of stacking gel in a beacker and mix with 10% AP and 1% TEMED. Pour out the water in the first step and pipet the stacking gel solution into the gap and insert the comb. Allow 20-30min to let it ...

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  • Native-PAGE - Assay-Protocol

    Single cell western blotting. A new approach to study protein expression on a cell-by-cell basis. Almost all cells studied in vitro and in vivo, including stem cells and cancer cells, do not represent a homogenous population but are inherently heterogeneous at a molecular level. This determines cellular identity, location, and function within a ...

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  • Calculate Polyacrylamide gel recipes for SDS-PAGE

    2011-11-3 · Calculate Polyacrylamide gel recipes for SDS-PAGE. Just enter the number of gels (18x16mm) and the percent polyacrylamide needed : Enter the number of gels: Enter Desired Percent: % ml: Total Volume : ml: ddH2O : ml: Acrylamide : ml: 1.5M Tris …

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  • Precast Polyacrylamide Gels 4-20% polyacrylamide |

    Precast Polyacrylamide Gels 4-20% polyacrylamide; find Sigma-Aldrich-P5466 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.

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  • SDS-PAGE Protein Loading Buffer 2X (Reducing)

    AR0131-20 Form: Liquid Size: 20mL: Contents: 4% SDS, 20% glycerol, 200mM DTT, 0.01% bromphenol blue and 0.1 M Tris HCl, pH 6.8 Description: SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system ...

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  • polyacrylamide, polyacrylamide Suppliers and

    Polyacrylamide polymer msds Polyacrylamide is a kind of linear polymers,it can soluble in water,insoluble in most organic solvents. According to the product features can be divided into:anionic polyacrylamide,cationic polyacrylamide,and non-ionic polyacrylamide. it is widely used in oil recovery,mineral processing,coal washing,metallurgy ...

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  • SDS-PAGE

    2003-8-18 · 20% Glycerol 8 ml 144.0 g Glycine 4% SDS 20% 8 ml 10.0 g SDS 10% ß-Mercaptoethanol 4 ml 0.5 mg/ml Bromophenol Blue 20 mg Dissolve and bring total volume to 1,000 ml with DDI H 2 O 15 ml deionized water. ... Use the 2 gel recipe for 1.5 mm thick gels. Title: SDS-PAGE …

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  • Electrophoresis for western blot | Abcam

    Polyacrylamide gels are formed from the polymerization of two compounds, acrylamide and N,N'-methylenebisacrylamide (bis, for short). Bis is a crosslinking agent for the gels. ... Load 20–40 µg total protein per mini-gel well. The gels should be submerged in migration buffer normally containing SDS, except in native gel electrophoresis.

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  • Use of the APS and TEMED in SDS-PAGE -

    TBE buffer. Prepare a 5X stock solution in 1 L of H 2 O: 20 mL of 0.5 M EDTA (pH 8.0) The 0.5X working solution is 45 mM Tris-borate/1 mM EDTA. TBE is usually made and stored as a 5X or 10X stock solution. The pH of the concentrated stock buffer should be ~8.3. Dilute the concentrated stock buffer just before use and make the gel solution and ...

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  • TBE buffer - CSH Protocols

    2019-7-31 · Allow 20-30min for a complete gelation. 2. You can prepare the stacking gel solution while the separating gel is gelating. Prepare appropriate amount of stacking gel in a beacker and mix with 10% AP and 1% TEMED. Pour out the water in the first step and pipet the stacking gel solution into the gap and insert the comb. Allow 20-30min to let it ...

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  • Preparing SDS gels - Rice University

    Polyacrylamide gels are formed from the polymerization of two compounds, acrylamide and N,N'-methylenebisacrylamide (bis, for short). Bis is a crosslinking agent for the gels. ... Load 20–40 µg total protein per mini-gel well. The gels should be submerged in migration buffer normally containing SDS, except in native gel electrophoresis.

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  • Electrophoresis for western blot | Abcam

    2003-8-18 · 20% Glycerol 8 ml 144.0 g Glycine 4% SDS 20% 8 ml 10.0 g SDS 10% ß-Mercaptoethanol 4 ml 0.5 mg/ml Bromophenol Blue 20 mg Dissolve and bring total volume to 1,000 ml with DDI H 2 O 15 ml deionized water. ... Use the 2 gel recipe for 1.5 mm thick gels. Title: SDS-PAGE …

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  • SDS-PAGE

    Gel Loading Dye, Purple (6X) is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. The two dyes separate upon gel electrophoresis; the red band is the major indicator and migrates ...

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  • DNA Gel Loading Dye | NEB

    2014-12-23 · Actually the time varies from 20 min to 1 h, but I personally leave the gel to polymerize longer. Unpolymerized Acrylamide could build adducts with some proteins which could result in multiple bands. In addition acrymamide itself is toxic, so I always give it additional time to polymerize. Good luck!

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  • molecular biology - What is SDS PAGE gel

    Polyacrylamide | Tradeasia International. Brief Overview. Polyacrylamide (PAM) is a water-soluble polymer made up of acrylamide subunits. It has a unique effect to increase the viscosity of water or promote the flocculation of particles present in water, it can …

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  • polyacrylamide viscosity in algeria

    We recently demonstrated that patterned Parylene C films could be effectively used as a mask for directly copolymerizing proteins on polyacrylamide hydrogel (PAm). In this work, we have proved the ...

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  • Effect of patterned polyacrylamide hydrogel on

    Polyacrylamide Market size, share, trends, forecast and analysis report to 2027 segmented By major countries, types, applications and manufacturers including: SNF Group. PetroChina Daqing....

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  • Polyacrylamide Market Size 2021: In-Depth Industry ...

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    1.Safe and reliable: using food contact grade safety silica gel, it can be in direct contact with food, 2.Clear details: Durable silicone material, so that you can get clear details every time you bake, even baked into brown 3.High-temperature non-melting: Use high-quality silica gel material with a temperature resistance range of -20°C to 220°C.

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