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cationic polyacrylamide gradient gel electrophoresis distributors

  • Steady-state electrophoresis of RNA against a

    The typical gel formulations consist of 4% T polyacrylamide (3.3% cross linker) in the presence of a gradient of positively charged ions, i.e. basic Immobilines. We have tried different basic species, but the preferred gel formulation adopts the pK 10.3 and 12 Immobilines, since these species will be fully protonated at the standard running pH (0.12 M Tris/acetate pH 6.4).

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  • Steady-state electrophoresis of RNA against a

    A novel method for separation of RNA fragments is reported here, based on migrating the polyanionic RNA fragments in a polycationic polyacrylamide gel, made by incorporating positively charged monomers (the Immobilines used for creating immobilized pH gradients) into the neutral polyacrylamide backbone.

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  • Distributors | Cleaver Scientific

    2015-11-16 · Sterile Workspace for Preparation of RNA & DNA Samples. November 16, 2015. Cleaver Scientific has supplied a UV Sterilization Cabinet to Touchlight Genetics (Hampton, UK) who had sought a sterile workspace for the preparation of their RNA.

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  • Steady‐state electrophoresis of RNA against a

    Steady‐state electrophoresis of RNA against a gradient of cationic charges in a polyacrylamide matrix

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  • polyacrylamide gel - offers from polyacrylamide gel ...

    polyacrylamide gel Manufacturers Directory 3 million global importers and exporters polyacrylamide gel suppliers, manufacturers, wholesalers, polyacrylamide gel sellers, traders, exporters and distributors from China and around the world at EC21.com

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  • Steady‐state electrophoresis of RNA against a

    Thus, 2‐D runs in cationic matrices might be exploited for structural studies of RNA molecules. A novel method for separation of RNA fragments is reported here, based on migrating the polyanionic RNA fragments in a polycationic polyacrylamide gel, made by incorporating positively charged monomers (the Immobilines used for creating immobilized pH gradients) into the neutral polyacrylamide backbone.

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  • Decolorization and degradation of cationic red X-GRL

    The bacterial community in the UBF at its stable state was investigated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that all color and the majority of chemical oxygen demand (COD) (92-74%) can be removed as …

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    Polyacrylamide gel electrophoresis (PAGE) is a highly reliable and widely used technique for the separation, identification and characterization of proteins and protein mixtures. Although two-dimensional (2D)-PAGE, which combines protein isoelectric focusing (IEF) in the first dimension with sodium dodecyl sulfate (SDS)-PAGE molecular sieving in the second dimension, provides the highest resolution allowing one to separate 1000–2000 individual polypeptide spots on a single gel…

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  • [PDF] Polyacrylamide Gel Electrophoresis | Semantic

    Science. Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...

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  • The isoinhibitors of chymotrypsin/elastase from

    The other four isoinhibitors are cationic on electrophoresis at pH 9.3, separable from each other, and identical with those reported previously [R.J. Peanasky and G. M. Abu-Erreish (1971) in Proceedings International Research Conference on Proteinase Inhibitors (Fritz, H., and Tschesche, H., eds.), pp. 281-293, de Gruyter, New York].

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  • Distributors | Cleaver Scientific

    2015-11-16 · Polyacrylamide Gel Electrophoresis; Western Blotting ... to Showcase Electrophoresis Portfolio at Arablab 2016 Cleaver Scientific will be displaying its latest innovations in Gel Electrophoresis at ARABLAB 2016, to be ... 2016 The next generation OmniPAGE VS20WAVE-DGGE from Cleaver Scientific is a flexible Denaturing Gradient Gel ...

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  • Steady-state electrophoresis of RNA against a

    Steady-state electrophoresis of RNA against a gradient of cationic charges in a polyacrylamide matrix Electrophoresis . 2009 Nov;30(21):3696-700. doi: 10.1002/elps.200900321.

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  • Gel Electrophoresis of Proteins - GBV

    2013-3-1 · Non-denaturing polyacrylamide gel electrophoresis 35 ... Transverse gradient gel electrophoresis 42. Tricine SDS-polyacrylamide gel electrophoresis 46 Use of cationic detergents for PAGE 47 Acknowledgements 49 References 50 2. Protein detection methods 53 …

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  • polyacrylamide gel - offers from polyacrylamide gel ...

    polyacrylamide gel Manufacturers Directory 3 million global importers and exporters polyacrylamide gel suppliers, manufacturers, wholesalers, polyacrylamide gel sellers, traders, exporters and distributors from China and around the world at EC21.com

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  • [12] Systems for polyacrylamide gel electrophoresis ...

    1984-1-1 · R.C. Allen, H.R. Maurer (Eds.), Electrophoresis and Isoelectric Focusing in Polyacrylamide Gel, de Gruyter, Berlin (1974)

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    P.J. Wirth, in Encyclopedia of Separation Science, 2000 Introduction. Polyacrylamide gel electrophoresis (PAGE) is a highly reliable and widely used technique for the separation, identification and characterization of proteins and protein mixtures. Although two-dimensional (2D)-PAGE, which combines protein isoelectric focusing (IEF) in the first dimension with sodium dodecyl sulfate (SDS)-PAGE ...

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  • The interaction between alpha-2-macroglobulin

    Abstract. On starch-gel or polyacrylamide-gel electrophoresis of human serum, a supernumerary zone of aspartate aminotransferase activity may be demonstrated, migrating with the slow α 2 protein zone. This appearance is due only to cationic aspartate aminotransferase, bound by α 2-macroglobulin.The binding is strongly potentiated by dilute borate buffers.

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  • [PDF] Polyacrylamide Gel Electrophoresis | Semantic

    Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over a wide range.

    Get Price
  • All you need to know when choosing a Gradient Gel

    2020-11-12 · Gradient gels are a great form of polyacrylamide gel to use during a western blot, especially for experiment which are focusing on the separations of proteins of similar molecular weight, e.g. Isomers. So what is a gradient gel? A gradient gel is a type of Stacking gel used for SDS-PAGE experiments but with a bit of a twist.

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  • Distributors | Cleaver Scientific

    2015-11-16 · Polyacrylamide Gel Electrophoresis; Western Blotting ... to Showcase Electrophoresis Portfolio at Arablab 2016 Cleaver Scientific will be displaying its latest innovations in Gel Electrophoresis at ARABLAB 2016, to be ... 2016 The next generation OmniPAGE VS20WAVE-DGGE from Cleaver Scientific is a flexible Denaturing Gradient Gel ...

    Get Price
  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    Polyacrylamide gels are characterized by two parameters: total monomer concentration (%T, in g/100 ml) and weight percentage of crosslinker (%C). By varying these two parameters, the pore size of the gel can be optimized to yield the best separation and resolution for the proteins of interest. %T indicates the relative pore size of the ...

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  • Steady-state electrophoresis of RNA against a

    Steady-state electrophoresis of RNA against a gradient of cationic charges in a polyacrylamide matrix. ... based on migrating the polyanionic RNA fragments in a polycationic polyacrylamide gel, made by incorporating positively charged monomers (the Immobilines used for creating immobilized pH gradients) into the neutral polyacrylamide backbone ...

    Get Price
  • determination of the molecular weight of

    Gradient polyacrylamide gel electrophoresis. The M r values of pharmaceutical heparins and low‐molecular‐weight (LMW) heparin derivatives were examined as part of a collaborative study to develop methods for their characterization. Standard methods of M r determination rely on gel permeation high‐performance liquid chromatography (HPLC).

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  • All you need to know when choosing a Gradient Gel

    2020-11-12 · Gradient gels are a great form of polyacrylamide gel to use during a western blot, especially for experiment which are focusing on the separations of proteins of similar molecular weight, e.g. Isomers. So what is a gradient gel? A gradient gel is a type of Stacking gel used for SDS-PAGE experiments but with a bit of a twist.

    Get Price
  • Isolation of the contact regions of the neighboring ...

    was isolated from whole ribosomes by preparative polyacrylamide gel electrophoresis and subjected to cyanogen bromide cleavage. For effective cleavage non-oxidizing conditions had to be maintained throughout the preparation. A simple and generally applicable method of high-efficiency gel pre-electrophoresis with anionic and cationic thiols

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  • Polyacrylamide gel electrophoresis - Hyperleap

    SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field. These can be run in either agarose or polyacrylamide gels. Polyacrylamide is also often used in molecular biology applications as a ...

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  • Cytiva™ - Sigma-Aldrich

    Cytiva™ technologies have enabled breakthroughs in science and medicine that have shaped today's biotechnology industry. With a broad portfolio that includes Whatman ® filters and membranes, Amersham™ chromatography columns and resins, tissue culture reagents, and products for sample preparation, blotting, and protein analysis, Cytiva™ advances the discovery and manufacture of …

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  • GoPAGE™ 4-15 % TGN Mini, 10 unit(s) | Gradient

    Ready-to-use mini gels with 4-15 % polyacrylamide, for rapid and easy gel electrophoresis of proteins of 25 to 200 kDa. Gel cassette: 10 x 8,3 cm, 12 wells Precast mini gels for rapid and easy gel electrophoresis in laboratory and students courses.

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  • GoPAGE™ 4-15 % TGN Midi, 10 unit(s) | Gradient

    Ready-to-use mini gels with 4-15 % polyacrylamide, for rapid and easy gel electrophoresis of proteins of 25 to 200 kDa. Gel cassette: 10 x 10 cm, 12 wells Precast mini gels for rapid and easy gel electrophoresis in laboratory and students courses.

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  • SCIE-PLAS == PAGE Gel Reagents and Buffers

    Agarose Gel Reagents and Buffers PAGE Gel Reagents and Buffers SERVA supply a wide variety of reagents for polyacrylamide gel electrophoresis (PAGE), including acrylamide-bis solutions, molecular weight markers and all of the buffers, catalysts and initiators necessary to make native and denaturing polyacrylamide gels.

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  • SCIE-PLAS == Electrophoresis Chemicals

    PAGE Gel Reagents and Buffers SERVA supply a wide variety of reagents for polyacrylamide gel electrophoresis (PAGE), including acrylamide-bis solutions, molecular weight markers and all of the buffers, catalysts and initiators necessary to make native and denaturing polyacryla...

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  • determination of the molecular weight of

    Gradient polyacrylamide gel electrophoresis. The M r values of pharmaceutical heparins and low‐molecular‐weight (LMW) heparin derivatives were examined as part of a collaborative study to develop methods for their characterization. Standard methods of M r determination rely on gel permeation high‐performance liquid chromatography (HPLC).

    Get Price
  • Gradient Makers - Hoefer Inc

    The 500 ml SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base. An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers. Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns.

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  • Glycerol, ACS Grade | Gel Electrophoresis | AG

    In polyacrylamide gel electrophoresis, glycerol is used in sample preparation and gel formation. At a concentration of 5-10%, glycerol is used to increase the density of a sample so that it will layer at the bottom of a sample well. Additional uses include as an aid in casting gradient gels, a protein stabilizer, and storage buffer component.

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  • Cationization of Catalase, Peroxidase, and Superoxide ...

    2014-1-30 · mined by isoelectric focusing on a 5% polyacrylamide slab gel, using an ampholyte pHgradient from 3.5 to 9.5, according to the manufac-turer's instructions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Proteins wereseparated onan 8-16%polyacrylamide gradient slab gel according to the method ofLaemmli (26).

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  • Isolation of the contact regions of the neighboring ...

    was isolated from whole ribosomes by preparative polyacrylamide gel electrophoresis and subjected to cyanogen bromide cleavage. For effective cleavage non-oxidizing conditions had to be maintained throughout the preparation. A simple and generally applicable method of high-efficiency gel pre-electrophoresis with anionic and cationic thiols

    Get Price
  • Polyacrylamide gel electrophoresis - Hyperleap

    SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a variant of polyacrylamide gel electrophoresis, an analytical method in biochemistry for the separation of charged molecules in mixtures by their molecular masses in an electric field. These can be run in either agarose or polyacrylamide gels. Polyacrylamide is also often used in molecular biology applications as a ...

    Get Price
  • SCIE-PLAS == Electrophoresis Chemicals

    PAGE Gel Reagents and Buffers SERVA supply a wide variety of reagents for polyacrylamide gel electrophoresis (PAGE), including acrylamide-bis solutions, molecular weight markers and all of the buffers, catalysts and initiators necessary to make native and denaturing polyacryla...

    Get Price
  • Enzyme Purification by Electrophoresis - Creative

    Ready-to-use mini gels with 4-15 % polyacrylamide, for rapid and easy gel electrophoresis of proteins of 25 to 200 kDa. Gel cassette: 10 x 10 cm, 12 wells Precast mini gels for rapid and easy gel electrophoresis in laboratory and students courses.

    Get Price
  • GoPAGE™ 4-15 % TGN Midi, 10 unit(s) | Gradient

    Agarose Gel Reagents and Buffers PAGE Gel Reagents and Buffers SERVA supply a wide variety of reagents for polyacrylamide gel electrophoresis (PAGE), including acrylamide-bis solutions, molecular weight markers and all of the buffers, catalysts and initiators necessary to make native and denaturing polyacrylamide gels.

    Get Price
  • SCIE-PLAS == PAGE Gel Reagents and Buffers

    2012-7-23 · Gradient gels are cast with a higher concentration of acrylamide at the bottom than the top. Gradient gel applications include the determination of protein molecular weights and the separation of molecules which co-migrate on uniform gels. Casting of gradient gels requires a gradient forming apparatus, and is more labor intensive than casting uniform percentage gels.

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  • Casting Gradient Gels | National Diagnostics

    The 500 ml SG500 Gradient Maker is made of two cylindrical acrylic chambers joined and mounted on a flat acrylic base. An easy-to-use push-pull valve opens and closes the passage between the reservoir and mixing chambers. Suitable for forming polyacrylamide gradients or buffer gradients used with medium to small chromatography columns.

    Get Price
  • Gradient Makers - Hoefer Inc

    2020-11-5 · Disc Gel Electrophoresis in Polyacrylamide Gel--The cationic system of Reisfeld et al. (22), as modified by Denny et al. (23), was employed with some additional modifications. In place of ammonium persulfate as catalyst, riboflavin was mixed in the running gel to a final concentration of 0.00057c. Since ribo-

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  • Purification and Some Physicochemical Properties of ...

    Total Solutions. As an innovative state of the art whole column imaging detection CIEF (iCIEF) system provider, Advanced Electrophoresis Solutions Ltd. (AES) …

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  • Distributors Network - Bioreagent

    With main products Hyaluronic Acid, Hydrolyzed Collagen, Cationic Conditioner Polymers, and Pullulan, we are proud to supply high quality ingredients and full technical. consultant service to cosmetics, nutraceuticals and foods industry. In 2009, Joyvo joined in WeiKem group, which.

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  • Casting Gradient Gels | National Diagnostics

    2012-7-23 · Gradient gels are cast with a higher concentration of acrylamide at the bottom than the top. Gradient gel applications include the determination of protein molecular weights and the separation of molecules which co-migrate on uniform gels. Casting of gradient gels requires a gradient forming apparatus, and is more labor intensive than casting uniform percentage gels.

    Get Price
  • Electrophoresis | Products Category | Welcome to

    DGGE - Denaturing Gradient Gel Electrophoresis The VS20-DGGE is a complete system for DNA mutation analysis. Using the innovative vertical screw-clamp technology of the VS20-WAVE system ( makes Gradient Gel making very easy ), it is fully equipped with temperature control unit, stirrer, gradient mixer and casting accessories- to perform ...

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  • How to cast and run a Polyacrylamide Gel in the Wave

    2017-4-8 · The new VS20 WAVE Maxi System is Cleaver Scientifics latest product innovation for large format vertical gel electrophoresis. It’s designed to perform a variety of separations, including first and second-dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis, plus capillary tube gel IEF and electroblotting and is one of the most versatile maxi ...

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  • Impacts of Polyacrylamide Concentration on Protein

    2021-6-4 · Impacts of Polyacrylamide Concentration on Protein Experiments. Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE). Many scientists take advantage of these techniques for their research ...

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  • Agents & Distributors - Alzo International

    2021-7-12 · Serving the World – Alzo Authorized Agents and Distributors. Alzo International has a global sales and distribution network with offices in 40 countries servicing 6 continents. Africa. Asia. Australia. Canada. Central & South America. Europe. United States.

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  • Distributors for SciGene Products - AlphaMetrix

    Great Britain: Great Britain (FISH and G-Banding products only) Czech Republic, Slovac Republic: Stretton Scientific Ltd Tel.: +44 (0) 1246 862951 Mob: +44 (0) 7793 651386

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  • Sol-Gel : Distributors, manufacturers of Sol-Gel

    2018-5-11 · Sol-Gel : Distributors, manufacturers of Sol-Gel Chemical precursors and equipments. Sol-Gel. Distributors-Manufacturers of Sol-Gel related Chemical precursors. Silicates. Silica & Colloidales solutions. Silanes & Alkoxides. Nanoparticles.

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  • Distributors - chromres.com

    Chromatography Research Supplies + 1.502.491.6300 International. 1.800.327.3800 U.S. Only. [email protected]

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  • Halo Gel - South Africa's leading Gel Nail Polish

    Halo Gel Polish is the leading Gel Nail Polish distributor in Durban, South Africa. Our gel is manufactured from the finest materials and we offer a variety of the latest trending colours in the industry. Compared to other polishes, our Gel Polish is environmentally friendly, non-toxic and is free from animal ingredients.

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  • Asia Pacific Distributors – Your local PPE supplier

    Asia Pacific Distributors mission is to source the best products. We are a global company based in Australia that negotiates with supplies worldwide. We value our long term relationships with supplies and customers. Your trusted PPE supplier for 25 years. There is no product that we can’t source.

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  • Distributors | MicruX

    Mora-Garay Industrial Park · Building #6 · Juan de la Cierva, 2C, 33211 Gijón (Asturias), Spain; Phone/Fax: (+34) 984 151 019; Email: [email protected]

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  • Distributors - BullGuard

    Distributors. BullGuard products are available world-wide through our network of selected Distributors. You can make a purchase direct with your distributor of choice.

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  • CBC Distributors - Welcome

    CBC Distributors - Welcome. We are open for business and orders can be placed online – by phone, email or post. If you have any queries or special requests, please don’t hesitate to ring our sales team. Catalogues can all be viewed online. NEW PRODUCT - NOW AVAILABLE FROM STOCK.

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  • LI-COR Biosciences Biotechnology Distributors

    LI-COR Biotechnology Distributors. LI-COR Biotechnology products are used in more than 100 countries and are supported by a global network of distributors outside the U.S. Please contact the distributor in your country for product information and technical assistance.

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  • Welcome to AKZENTZ | International

    2021-7-13 · B&BEA CO., LTD 1035, No 11, Lane225, Jin Xiang Rd., Pu Dong New Dist. Shanghai, China Phone: +86 185 0167 9856 Phone: +86 135 2425 4158 E-mail: [email protected]

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  • Welcome to Control Distributors

    Inspections, installations, upgrades, retrofits, mobile machining, valve repacking and reconditioning, component… Learn more…

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  • Interport Distributors

    Interport Distributors is strategically located in South Florida in order to efficiently serve our customers throughout various countries in Latin America. Int’l Duty Free Retail. Partnering with some of the most prestigious Duty Free operators in the industry. Consumer Electronics. We are proud to offer the latest in consumer electronics ...

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  • EFDA

    Welcome. EFDA represents the interests of fastener distributors at European and global level. We bring together active and established national associations and a growing number of independent distributors in other European countries. In total 170 innovative and top-performing associates have a massive combined inventory of fasteners and other ...

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