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making process of 8 cationic polyacrylamide gel recipe

  • Effect of cationic polyacrylamide on the processing

    2015-6-1 · By lowering the gel point, we significantly opened the structure of the flocs to reduce the drainage time by forming a more porous film. Nanocellulose films properties and process were optimized by flocculating cellulosic fibres with high charge (50%) and high molecular weight CPAM (15 MDa). Drainage improvement likely resulted from full flocculation of all nanofibres into less compressible …

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  • Preparation of cationic polyacrylamide by aqueous two ...

    Cationic polyacrylamide (CPAM) was synthesized by aqueous two-phase polymerization technique using acry-lamide (AM) and dimethylaminoethyl methacrylate methyl chloride (DMC) as raw materials, aqueous polyethylene glycol 20000 (PEG 20000) solution as dispersant, 2,2′-azobis(2-amidinopropane) dihydrochloride (V-50) as initiator and

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  • DNA Polyacrylamide Gel Electrophoresis

    2013-11-4 · DNA Polyacrylamide Gel Electrophoresis How to pour and run a neutral polyacrylamide gel. Buffers and Solutions Acrylamide:bisacrylamide (29:1) (30% w/v) Ammonium persulfate (10% w/v) Ammonium persulfate is used as a catalyst for the copolymerization of acrylamide and bisacrylamide gels. The polymerization reaction is driven by free

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  • Denaturing Polyacrylamide Gel Electrophoresis

    2019-2-5 · polyacrylamide around gel. 19. Hold two pieces of dry 46 × 57–cm blotting paper together as one piece. Beginning at one end of gel and working slowly towards the other, lay paper on top of gel. Take care to prevent air bubbles from forming between paper and gel. 20. Peel blotting paper up; gel should come off plate with it. Gradually curl paper and

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · a small-pore resolving gel, Figure 2.2) and different buffers in the gels and electrode solutions (Wheeler et al. 2004) In gel electrophoresis, proteins do not all enter the gel matrix at the same time. Samples are loaded into wells, and the proteins that are closer to the gel enter the gel first. In continuous systems, the uniform

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    Industrial sewage dewatering treatment, cationic polyacrylamide, flocculant. Main Production: 1. Oilfield Chemicals, Oilfield chemical is a general germ,The oilfield chemicals we can supply include anionic polyacrylamide used for oil drilling mud additives , drag reduction agent and EOR(enhanced oil recovery)agent . 2.

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  • cationic polyacrylamide flocculant , China cationic ...

    1,591 polyacrylamide gel recipe products are offered for sale by suppliers on Alibaba.com A wide variety of polyacrylamide gel recipe options are available to you, There are 2 suppliers who sells polyacrylamide gel recipe on Alibaba.com, mainly located in Asia.

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  • polyacrylamide gel recipe, polyacrylamide gel recipe ...

    Alibaba.com offers 4,496 textile chemical cationic polyacrylamide products. A wide variety of textile chemical cationic polyacrylamide options are available to you, such as classification, usage, and type.

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  • Preparation of cationic polyacrylamide by aqueous two ...

    monomers concentration 8~15%, PEG 20000 concentration 15~25%, and PDMC concentration 0.5~1.5%. Finally, the for-mation process of copolymer particles was investigated by optical microscope. Keywords: polymer synthesis, aqueous two-phase polymerization, cationic polyacrylamide, dimethylaminoethyl methacry-late methyl chloride, polyethylene ...

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  • Calculate Polyacrylamide gel recipes for SDS-PAGE

    2011-11-3 · Calculate Polyacrylamide gel recipes for SDS-PAGE. Just enter the number of gels (18x16mm) and the percent polyacrylamide needed : Enter the number of gels: Enter Desired Percent: % ml: Total Volume : ml: ddH2O : ml: Acrylamide : ml: 1.5M Tris pH 8.8 …

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  • Preparation of protein samples for SDS-polyacrylamide

    2006-6-25 · HCl/Tris-glycine, and pH, 6.8/8.3, respec-tively (5, 6). Discontinuous buffer systems allow larger sample volumes to be loaded while maintaining good resolution of sample components because the proteins are fo-cused, or “stacked,” as thin bands prior to entering the resolving gel. Second was the use of the detergent sodium dodecyl sulfate

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  • Polyacrylamide precast gels for electrophoresis,

    The most common buffer system for using agarose and polyacrylamide as the gel material in the electrophoresis separation of DNA is a continuous buffer system of around pH 7.8 to 8.3, in which a gel buffer solution is equal in composition with an electrophoresis buffer solution: a tris-acetic acid buffer, contracted to “TAE” herein, which ...

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  • Polyacrylamide Emulsions Handbook - SNF

    2012-7-22 · Polyacrylamide Emulsions Handbook 2-2 Storage and handling of emulsions : basic principles Emulsions must be stored inside a building at a constant temperature between 5°C and 30°C. During the storage and handling, the emulsion must not be contaminated by water.

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  • POLYACRYLAMIDE GEL FOR USE WITH TRADITIONAL

    20030051995: Plating device and plating method: March, 2003: Nobata et al. 20090101507: SPERM CELL SEPARATION BY ELECTROPHORESIS: April, 2009: Aitken et al.

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    SDS-PAGE Gel. 1. Prepare the separation gel (10%). Mix in the following order: After adding TEMED and APS to the SDS-PAGE separation gel solution, the gel will polymerize quickly, so add these two reagents when ready to pour. 2. Pour gel, leaving ∼2 cm below the bottom of the comb for the stacking gel. Make sure to remove bubbles.

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  • SDS-PAGE Gel - CSH Protocols

    2020-4-9 · Cold process: The cold process is the most common method of making soap from scratch using oils and lye. Hot process: A variation of the cold process method, the hot process requires cooking the soap is actually cooked in a slow cooker or oven. Rebatching: This method grinds up and re-blends batches of poorly made homemade soap.

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  • Cationic polyacrylamide. Top Hóa chất bán lẻ, hóa chất

    Application of SPECFLOC C1492. One of the largest uses for polyacrylamide is to flocculate solids in a liquid. This process applies to water treatment, and processes like paper making and screen printing. Polyacrylamide can be supplied in a powder or liquid form, with the liquid form being subcategorized as solution and emulsion polymer.

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  • cationic polyacrylamide powder and emulsion in

    2021-7-7 · Cationic Polyacrylamide Flocculant Powder - Sinofloc Supplier. The polyacrylamide is a synthetic cationic polymerization flocculants and so mainly related to chain length and the charge of the dewatering performance of polyacrylamide excessive dosage, the positive charge on the ion polyacrylamide adsorption, due to electrostatic repulsion, it will reduce the sludge settling …

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  • Polyacrylamide gel method: synthesis and property of

    2010-9-24 · Effects of monomer (AM) concentration, monomer/crosslinker (AM/MBAM) ratio and salt concentration on the thermal behavior of precursor gel and the properties of BeO nanopowder synthesized by polyacrylamide gel method were investigated. The decomposition process of precursor gel was also studied. The decomposition process of precursor gel is that, first, the extraction of free …

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a monobasic acid, titrated to a specified pH range. With the gels constituted as stated earlier, proteins can be successfully subjected to electrophoresis using the commonly available Laemmli's buffer ...

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  • US6726821B1 - Polyacrylamide precast gels for ...

    High quality Bluwat Cationic Polyacrylamide Flocculant Flocking Agent Water Treatment from China, China's leading cationic polyelectrolyte flocculants product, with strict quality control industrial water treatment chemicals factories, producing high quality industrial water treatment chemicals products.

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  • Bluwat Cationic Polyacrylamide Flocculant Flocking

    2015-2-2 · Pour off the n-butanol from the polymerized Separating Gel, wash the gel top with water, and fill the gap remaining in the chamber with the Stacking Gel mixture. Insert the comb. 10. Polymerize the acrylamide for 1 hour. 11. When the Stacking Gel has polymerized, remove the comb without distorting the shapes of the well. Remove the

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  • Molecular Techniques and Methods Native Gel

    2018-4-4 · 11/15/2017 5 What is Anionic Polyacrylamide? • One of the most common polymer flocculants on the market • Common uses of PAM as a flocculant: reduction of sediment and nutrient loads to natural lakes and ponds wastewater and drinking water treatment clarification of effluents in industries like pulp & paper, aquaculture • Also popular for erosion prevention in irrigation furrows and on

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  • Best Practices Guidance for the Use of Anionic ...

    The present invention relates to polysaccharide, preferably starch, of high purity having cationic degree of substitution over 0.50 and bound nitrogen index after cationisation reaction stage over 0.75, and to preparation thereof as well as to its use in making of paper or paperboard.

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  • WO2007121981A1 - Cationic polysaccharide, its

    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.

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  • A cationic polyacrylamide dispersion synthesis by ...

    2021-7-7 · Full Article. A CATIONIC POLYACRYLAMIDE DISPERSION SYNTHESIS BY DISPERSION POLYMERIZATION IN AQUEOUS SOLUTION. Yufeng Wang, a,b, * Kefu Chen, a Lihuan Mo, a,* and Huiren Hu c A cationic polyacrylamide (CPAM) dispersion, the copolymer of acrylamide (AM) and acryloyloxyethyltrimethyl ammonium chloride (DAC), has been synthesized through dispersion …

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  • The principle and method of polyacrylamide gel ...

    Preparation of polyacrylamide gel An example performed at MBL Step-by-step procedure; Gather combs, glass plates, spacer (silicone tubing), and binder clips. A comb is used to make wells (lanes) to load samples. Use an appropriate comb depending on the sample size. Example: Use an 8-lane comb for 7 samples and molecular weight markers. ...

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  • wastewater treatment chemicals cationic

    2021-6-17 · chemicals used in water treatment--cationic and anionic. chemicals used in water treatment--cationic and anionic polyacrylamide Anionic polyacrylamide: It is widely used in waste water treatment of chemical industry, municipal sewage treatment, such as coal washing, mineral processing, metallurgy, iron and steel industry and electronic industry.

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  • Polyacrylamide gel method: synthesis and property of

    2010-9-24 · Effects of monomer (AM) concentration, monomer/crosslinker (AM/MBAM) ratio and salt concentration on the thermal behavior of precursor gel and the properties of BeO nanopowder synthesized by polyacrylamide gel method were investigated. The decomposition process of precursor gel was also studied. The decomposition process of precursor gel is that, first, the extraction of free …

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  • White Water Cleaning Chemicals Cationic

    Polyacrylamide (IUPAC poly (2-propenamide) or poly (1-carbamoylethylene), abbreviated as PAM) is a polymer (-CH2CHCONH2-) formed from acrylamide subunits. It is highly water-absorbent, forming a soft gel when hydrated, used in such applications as polyacrylamide gel electrophoresis, and can also be called ghost crystals when cross-linked, and ...

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  • BASIC PROTOCOL: PURIFICATION OF

    2016-8-9 · A 20-mer oligonucleotide is typically recovered in a 80 % yieldafter 3 hours of rotary shaking thereby making this technique comparableto electroelution. Since elution isa diffusion-controlled process, more buffer will aid in elution efficiency.Also, note that longer oligonucleotides will take longer to diffuse fromthe gel.

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  • US6726821B1 - Polyacrylamide precast gels for ...

    A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a monobasic acid, titrated to a specified pH range. With the gels constituted as stated earlier, proteins can be successfully subjected to electrophoresis using the commonly available Laemmli's buffer ...

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  • Molecular Techniques and Methods Native Gel

    2015-2-2 · Pour off the n-butanol from the polymerized Separating Gel, wash the gel top with water, and fill the gap remaining in the chamber with the Stacking Gel mixture. Insert the comb. 10. Polymerize the acrylamide for 1 hour. 11. When the Stacking Gel has polymerized, remove the comb without distorting the shapes of the well. Remove the

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  • How to Make Aloe Vera Gel: 8 Steps (with Pictures) -

    Beginning the process with the necessary moonshine ingredients can make a huge difference in the final product. Below is an overview of the moonshine making process: Procedure. Collect your moonshine ingredients and materials, including: 5 gallons of water; 8.5 pounds of cracked corn maize; 1.5 pounds of grounded malted barley; Distillers yeast

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  • The principle and method of polyacrylamide gel ...

    Preparation of polyacrylamide gel An example performed at MBL Step-by-step procedure; Gather combs, glass plates, spacer (silicone tubing), and binder clips. A comb is used to make wells (lanes) to load samples. Use an appropriate comb depending on the sample size. Example: Use an 8-lane comb for 7 samples and molecular weight markers. ...

    Get Price
  • Preparing SDS gels - Rice University

    Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the proteins move in response to the electric field. Proteins contain an overall positive or negative charge; this enables the movement of a protein ...

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  • Introduction to SDS-PAGE - Separation of Proteins

    A copolymer of acrylamide (AM) with acryloyloxyethyl trimethyl ammonium chloride (DAC) as the cationic monomer was synthesized under the irradiation of high-pressure mercury lamp with 2,2-azobis(2-amidinopropane) dihydrochloride (V-50) as the photoinitiator. The compositions of the photoinduced copolymer were characterized by Fourier transform infrared spectra (FTIR), ultraviolet spectra (UV ...

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  • Photoinitiated Polymerization of Cationic Acrylamide

    Protein polyacrylamide gel electrophoresis: (1)The basic principle of SDS denaturing in-continuous polyacrylamide gel electrophoresis is based on differences in the molecular weight of the protein, SDS is an anionic surfactant, capable of binding with the hydrophobic portion of the protein thus making the protein bring a large number of anions ...

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  • Cas 9003-05-8,Polyacrylamide | lookchem

    2012-7-23 · Cationic flocculantsthat bring cationic charges (+) into the medium. Different levels (percentages) of ionic chargeare available : from 0 to 100%. Several commercial forms exist and they come from differents methods of polymerization : Powders :The monomers are polymerized in a gel form. The gel obtained is then ground and dried.

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  • PREPARATION OF ORGANIC POLYMERS - SNF

    2016-8-9 · A 20-mer oligonucleotide is typically recovered in a 80 % yieldafter 3 hours of rotary shaking thereby making this technique comparableto electroelution. Since elution isa diffusion-controlled process, more buffer will aid in elution efficiency.Also, note that longer oligonucleotides will take longer to diffuse fromthe gel.

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  • BASIC PROTOCOL: PURIFICATION OF

    For example, if you are making a 30mL gel that you want to be 0.8%, the amount of agarose to use is 0.24g. Measure out the agarose using wax weighing paper. We keep some agarose in a scintillation vial near the balance; that way you don’t need a spatula to measure it …

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  • How to make an agarose gel for electrophoresis

    Blue native polyacrylamide gel elec- trophoresis (BN-PAGE) is a separation method with a higher resolution than gel filtration or sucrose density ultra- centrifugation that can be used to analyze abundant, stable MPCs from 10 kD to 10 MD.

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  • Blue Native Polyacrylamide Gel Electrophoresis (BN

    2020-5-27 · 2 L of loading bu er and then added into the gel. The first five lanes in each gel were experimental samples from di erent incubation times and with nanoparticles, and the last five lanes were used for control (same incubation time but without nanoparticles). PAGE conditions: 80 V, 120 min., 20% gel …

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  • Gel Phase in Soap Making – Preventing and Forcing

    2016-10-3 · Forcing Gel Phase in Soap Making. There are many times in soap making that you will actually want to force your soap through gel phase. It might be because you are tired of partial gel, or it might be that you are soaping with natural colorants so you want to force gel to get vibrant colors. Here are some tips for forcing gel phase. Soap warmer.

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  • Principle and Protocol of Sodium Dodecyl Sulphate ...

    2015-11-17 · Inject the separating gel into the gap of the two glass sheets quickly, leaving space for the infusion of stacking gel (comb teeth length plus 1cm); cover the separating gel with 0.1% SDS carefully( when the concentration of acrylamide ≤ 8%) or isobutanol or water (w hen the acrylamide concentration ≥10%); the cover layer can prevent the ...

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  • Instructions for Clear Glycerin Soap

    2011-10-14 · Base Part of Recipe These recipes are 40 oz. of oils. If you don’t want to make this much you can split recipe in half. The oils in these recipes will make your clearest soaps. Recipe 1: 20 oz. Lard or 50% of total oils 8 oz. Coconut Oil or 20% of total oils 12 oz. Castor Oil or 30% of total oils 15.2 oz. Distilled water 5.8 oz. lye

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  • Alginate/graphene double-network nanocomposite

    2020-3-29 · The following is reprinted with permission from The Big Book of Homemade Products for Your Skin, Health and Home by Jan Berry, Page Street Publishing Co. 2020. Photo credits: Jan Berry where noted. Soap-Making Basics. Before you jump into the process of making …

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  • How to Make Soap ~ Soap Making for Beginners

    Making the gel (for a 1% gel, 50 mL volume) Weigh out 0.5 g of agarose into a 250 mL conical flask. Add 50 mL of 0.5xTBE , swirl to mix. It is good to use a large container, as long as it fits in the microwave, because the agarose boils over easily. Microwave for about 1 minute to dissolve the agarose.

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  • Sugaring Hair Removal Recipe - LiveAbout

    99% Purity Chemical Polymers Polyacrylamide CAS NO. 9003-05-8 9003-05-8 Suppliers,provide 99% Purity Chemical Polymers Polyacrylamide CAS NO. 9003-05-8 9003-05-8 product and the products related with China (Mainland) 99% Purity Chemical Polymers Polyacrylamide CAS NO. 9003-05-8 9003-05-8 Hebei Bosang International Trade Co.,Ltd. China (Mainland)

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  • Agarose gel electrophoresis (basic method)

    You can prevent gel phase by putting soap in the freezer for 24 hours. Curing : Technically, cold process soap is safe to use after a few days. However, we recommend letting the soap cure for 4-6 weeks in a cool, dry place with good airflow. Excess water will evaporate, which creates harder and milder bars that last longer in the shower.

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  • Gel electrophoresis (article) | Khan Academy

    2013-9-20 · This process might have to be repeated two or three times to remove close to 100% of soaps. The second and third washings can be done with water alone. After the third washing any remaining water gets removed by re-heating the oil slowly (Figure 8), the water and other impurities sink to …

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  • Biodiesel recipe from Mike Pelly: Journey to Forever

    2019-4-8 · 1-2 drops essential oil (e.g., lavender, jojoba, chamomile) Rinse hair with rainwater (usually acidic) or make your own acidifying rinse by adding 2 tablespoons apple cider vinegar to an empty 20-ounce water bottle. Fill the remainder of the bottle with water and use the mixture to rinse clean hair.

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  • How Hair Detangler Works and Recipes to Make It

    2021-6-11 · Paper Making Process by SAPPI Tube. Papermaking at Gulf Paper Manufacturing Co.- Kuwait. Sack Kraft Paper Production by Mondi. The Making of Pulp at Clearwater Paper Corp. 'The Paper People' How Paper is Made (15 min) Tissue paper making line-1. Tissue paper making line-2. Tissue paper making line-3.

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