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making process of nonionic polyacrylamide gel recipe

  • Polyacrylamide (PAM) - DXD

    Polyacrylamide. Polyacrylamide production process: Acrylamide aqueous solution is used as a raw material, and polymerization reaction is performed under the action of an initiator. The polyacrylamide gel block generated after the reaction is cut, cut, granulated, dried, and pulverized. According to ionic characteristics, it can be divided into four ...

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  • Nonionic Polyacrylamide Flocculant - Sinoloc Supplier

    2019-2-5 · polyacrylamide around gel. 19. Hold two pieces of dry 46 × 57–cm blotting paper together as one piece. Beginning at one end of gel and working slowly towards the other, lay paper on top of gel. Take care to prevent air bubbles from forming between paper and gel. 20. Peel blotting paper up; gel should come off plate with it. Gradually curl paper and

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  • Denaturing Polyacrylamide Gel Electrophoresis

    2018-3-5 · a small-pore resolving gel, Figure 2.2) and different buffers in the gels and electrode solutions (Wheeler et al. 2004) In gel electrophoresis, proteins do not all enter the gel matrix at the same time. Samples are loaded into wells, and the proteins that are closer to the gel enter the gel first. In continuous systems, the uniform

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-10-1 · Nonionic polyacrylamide (NPAM) and phenolic resin cross-linker were used to prepare the phenolic resin gel. The hydrolysis degree and average molecular weight of NPAM were 3.31% and 9,650,000 g/mol, respectively. The cross-linker was prepared by using phenol, formaldehyde and urotropine, and it was purchased from Yuguang Co. Ltd., China.

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  • Study on rheology and microstructure of phenolic

    2015-11-17 · Inject the separating gel into the gap of the two glass sheets quickly, leaving space for the infusion of stacking gel (comb teeth length plus 1cm); cover the separating gel with 0.1% SDS carefully( when the concentration of acrylamide ≤ 8%) or isobutanol or water (w hen the acrylamide concentration ≥10%); the cover layer can prevent the ...

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    A preferred type of nonionic surfactant for use in accordance with the invention are ethoxylated long carbon chain alcohols. Typically, nonionic ethoxamers of this type have an eight to 20 carbon atom alkyl chain preferably 14 to 18 and an average of about four to …

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  • Principle and Protocol of Sodium Dodecyl Sulphate ...

    SDS-PAGE Gel. 1. Prepare the separation gel (10%). Mix in the following order: After adding TEMED and APS to the SDS-PAGE separation gel solution, the gel will polymerize quickly, so add these two reagents when ready to pour. 2. Pour gel, leaving ∼2 cm below the bottom of the comb for the stacking gel. Make sure to remove bubbles.

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  • Process for making a free flowing soap-nonionic

    2016-10-4 · For Making Special Brown Manufacturing Process Process Flow Diagram 18. Herbal Creams ... (Nonionic/Perfume) Toilet Bowl Cleaner Tub & Tile Cleaner Tub, Tile and Bathroom Cleaner ... Bath Gel Bubble-Bath Gel Blooming Bath Oil Bath and Shower Gel Bubble Bath - Premium Foam Bath

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  • Preparation of protein samples for SDS-polyacrylamide

    2006-6-25 · Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips Anthony C. Grabski 1and Richard R. Burgess2— Novagen, Inc. and 2McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706 continued from page 9 pensive method to maintain very low basal

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  • nonionic pam polyacrylamide - aladine.be

    Nonionic Polyacrylamide Pam Buy Nonionic. Nonionic Polyacrylamide Details:. PAM is a water-soluble polymer, the appearance of a white or yellowish powder, easy to absorb water, long-term thermal decomposition, the decomposition temperature above 200 ℃, under anaerobic conditions, 210 ℃ carbonized black powder.

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · a small-pore resolving gel, Figure 2.2) and different buffers in the gels and electrode solutions (Wheeler et al. 2004) In gel electrophoresis, proteins do not all enter the gel matrix at the same time. Samples are loaded into wells, and the proteins that are closer to the gel enter the gel first. In continuous systems, the uniform

    Get Price
  • SDS-PAGE Gel - CSH Protocols

    SDS-PAGE Gel. 1. Prepare the separation gel (10%). Mix in the following order: After adding TEMED and APS to the SDS-PAGE separation gel solution, the gel will polymerize quickly, so add these two reagents when ready to pour. 2. Pour gel, leaving ∼2 cm below the bottom of the comb for the stacking gel. Make sure to remove bubbles.

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  • machine for emulsion nonionic polyacrylamide in

    2021-7-8 · Saudi Arabia Polyacrylamide Market by Type, Opportunities. Saudi Arabia Polyacrylamide Market by Type (Anionic Polyacrylamide, Nonionic Polyacrylamide, Cationic Polyacrylamide and Others), By Form (Powder, Emulsion, Gel and Others), By Application (Wastewater Treatment, Oil & Gas Extraction, Mining, Paints & Coatings and Others) – Opportunities & Forecast, 2019-2026

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  • What Is Polyacrylamide Gel? (with pictures)

    2016-8-9 · A 20-mer oligonucleotide is typically recovered in a 80 % yieldafter 3 hours of rotary shaking thereby making this technique comparableto electroelution. Since elution isa diffusion-controlled process, more buffer will aid in elution efficiency.Also, note that longer oligonucleotides will take longer to diffuse fromthe gel.

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  • BASIC PROTOCOL: PURIFICATION OF

    2020-9-24 · 2. Heat the seeds in 2 cups (475 milliliters) of water. If you soaked the seeds, drain off any excess water. Pour 2 cups (475 milliliters) of fresh water into the pot, then bring it to a boil over high heat. Immediately reduce the heat to a simmer. 3. Cook the seeds until you get your desired consistency.

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  • How to Make a Flammable Gel or Jelly - ThoughtCo

    2020-4-9 · Cold process: The cold process is the most common method of making soap from scratch using oils and lye. Hot process: A variation of the cold process method, the hot process requires cooking the soap is actually cooked in a slow cooker or oven. Rebatching: This method grinds up and re-blends batches of poorly made homemade soap.

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  • nonionic pam polyacrylamide - aladine.be

    Nonionic Polyacrylamide Pam Buy Nonionic. Nonionic Polyacrylamide Details:. PAM is a water-soluble polymer, the appearance of a white or yellowish powder, easy to absorb water, long-term thermal decomposition, the decomposition temperature above 200 ℃, under anaerobic conditions, 210 ℃ carbonized black powder.

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  • Hand Casting Polyacrylamide Gels | LSR | Bio-Rad

    The glass plates must be clean and free of chips. Clean glass plates with ethanol and lint-free cloths before use. The height of the stacking gel should be at least 2x the height of the sample in the well. This ensures band sharpness, even for diluted protein samples. Store gels flat in the fridge at 4°C.

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  • Polyacrylamide precast gels for electrophoresis,

    Among the prior processes for manufacturing gels which are superior in shelf-life is a process for manufacturing a polyacrylamide gel having the increased shelf-life disclosed in Published Unexamined Patent Application in Japan No. H04-184 163, which involves a gel buffer solution composed of Tris, ampholyte and acid to be storable for a ...

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  • machine for emulsion nonionic polyacrylamide in

    2021-7-8 · Saudi Arabia Polyacrylamide Market by Type, Opportunities. Saudi Arabia Polyacrylamide Market by Type (Anionic Polyacrylamide, Nonionic Polyacrylamide, Cationic Polyacrylamide and Others), By Form (Powder, Emulsion, Gel and Others), By Application (Wastewater Treatment, Oil & Gas Extraction, Mining, Paints & Coatings and Others) – Opportunities & Forecast, 2019-2026

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  • polyacrylamide anionic nonionic powder emulsion

    Polyacrylamide Anionic Cationic Nonionic, Polyacrylamide. Alibaba offers 470 polyacrylamide anionic cationic nonionic products. About 52% of these are water treatment chemicals. A wide variety of polyacrylamide anionic cationic nonionic options are available to you, such as free samples.

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  • China Nonionic Polyacrymide Manufacturers and

    2021-7-9 · Getting started; Nonionic Polyacrymide; Nonionic Polyacrymide - China Manufacturers, Factory, Suppliers. We provide fantastic energy in top quality and advancement,merchandising,gross sales and marketing and operation for Nonionic Polyacrymide, 9003-05-8 Anionic Polyacrylamide, De Coloring Agent, Dadmac Used In Paper Making,Liquid 50 Polyamine.We're going to make higher …

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  • Toilet Paper Making Pocess Anionic Polyacrylamide

    2020-9-24 · 2. Heat the seeds in 2 cups (475 milliliters) of water. If you soaked the seeds, drain off any excess water. Pour 2 cups (475 milliliters) of fresh water into the pot, then bring it to a boil over high heat. Immediately reduce the heat to a simmer. 3. Cook the seeds until you get your desired consistency.

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  • Polyacrylamide gel electrophoresis - Alfa

    2016-7-22 · Polyacrylamide gel electrophoresis Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a free-radical polymerization that requires an initiator, usually ammonium persulfate, and a

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    Among the prior processes for manufacturing gels which are superior in shelf-life is a process for manufacturing a polyacrylamide gel having the increased shelf-life disclosed in Published Unexamined Patent Application in Japan No. H04-184 163, which involves a gel buffer solution composed of Tris, ampholyte and acid to be storable for a ...

    Get Price
  • Polyacrylamide precast gels for electrophoresis,

    2021-7-9 · Getting started; Nonionic Polyacrymide; Nonionic Polyacrymide - China Manufacturers, Factory, Suppliers. We provide fantastic energy in top quality and advancement,merchandising,gross sales and marketing and operation for Nonionic Polyacrymide, 9003-05-8 Anionic Polyacrylamide, De Coloring Agent, Dadmac Used In Paper Making,Liquid 50 Polyamine.We're going to make higher …

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  • China Nonionic Polyacrymide Manufacturers and

    Flocculants Nonionic Polyacrylamide For Water Clarification, Paper Processing, Drilling, Textile And Sludge Dewatering from China. 141 similar products are also available from Suppliers of Textiles & Leather Products.

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  • Flocculants Nonionic Polyacrylamide For Water ...

    Blue native polyacrylamide gel elec- trophoresis (BN-PAGE) is a separation method with a higher resolution than gel filtration or sucrose density ultra- centrifugation that can be used to analyze abundant, stable MPCs from 10 kD to 10 MD.

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  • Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE ...

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  • mixing barrel nonionic polyacrylamide in sri lanka ...

    drinking water treatment flocculant anionic polyacrylamide Polyacrylamide, abbr. PAM, is a kind of water soluble polymer, has unique effect to increase the visc

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  • drinking water treatment flocculant anionic

    2021-3-12 · The study deals with the synthesis and characterization of the hydrophobically modified polyacrylamide (HMPA) copolymer and its functional property evaluation in mixed polymer-gemini surfactant systems for application in enhanced oil recovery (EOR). The copolymer was initially prepared in the laboratory using acrylamide and N-phenylacrylamide monomer units via an addition …

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  • Characterization of Hydrophobically Modified ...

    A nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents. Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing.

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  • Nonionic Polyacrylamide - chinabypam.com

    2015-2-2 · 11. When the Stacking Gel has polymerized, remove the comb without distorting the shapes of the well. Remove the clips holding the plates together, and install the gel in the apparatus. 12. Fill apparatus with Reservoir Buffer. Push out the bottom spacer from the gel and remove bubbles from both the top and underneath of the gel. Use the gel ...

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  • Molecular Techniques and Methods Native Gel

    Flocculants Nonionic Polyacrylamide For Water Clarification, Paper Processing, Drilling, Textile And Sludge Dewatering from China. 141 similar products are also available from Suppliers of Textiles & Leather Products.

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  • Flocculants Nonionic Polyacrylamide For Water ...

    Blue native polyacrylamide gel elec- trophoresis (BN-PAGE) is a separation method with a higher resolution than gel filtration or sucrose density ultra- centrifugation that can be used to analyze abundant, stable MPCs from 10 kD to 10 MD.

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  • Blue Native Polyacrylamide Gel Electrophoresis (BN

    2. The process of claim 1 in which the water soluble polymer is a polyalkyleninime. 3. The process of claim 1 in which the anionic or nonionic polymer is polyacrylamide and the water soluble polymer is polyethylenimine. 4. A process for reducing the permeability of a subterranean formation traversed by a well bore which comprises:

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  • Reducing permeability of highly permeable zones in ...

    (57) Abstract: The method of dehydration and purification gel of polyacrylamide includes freezing, grinding and processing solvent. While remaining solvent from the dehydrated and purified particles are removed by a stream of air or inert gas at a temperature not exceeding 48 o C. expanding the number of solvents used for the dehydration and purification of the gel from impurities.

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  • The method of dehydration and purification of ...

    Hot process soap making will give you a different look to your beautiful bars. The entire curing time is much faster with this method, and your work can be ready to sell in as little as 24 hours. Remember, this is written with the beginner in mind. More advanced recipes can be found in the Hot Process Soap section of the Recipe Directory

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  • Lesson 3 : Hot Process Soap Making – Thermal

    Blend all these together for a few seconds with a spatula or hand blender. Pour the soap mixture into mold (s). Cover mold (s) with plastic wrap and cover/wrap these with towels for insulation. Let the soap set for 24 to 48 hours or so before taking it out of the mold (s). Unmold and cut the soap into bar.

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  • The principle and method of polyacrylamide gel ...

    Preparation of polyacrylamide gel An example performed at MBL Step-by-step procedure; Gather combs, glass plates, spacer (silicone tubing), and binder clips. A comb is used to make wells (lanes) to load samples. Use an appropriate comb depending on the sample size. Example: Use an 8-lane comb for 7 samples and molecular weight markers. ...

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  • Polyacrylamide Gel Electrophoresis - Alfa Aesar

    2016-3-23 · Polyacrylamide Gel Electrophoresis Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylene-bis-acrylamide, commonly called BIS. This process is a free-radical polymerization that requires an initiator, usually ammonium

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  • flour mixer to make small dough flocculation nonionic ...

    2021-7-12 · The application of nonionic polyacrylamide in building materials has been widely promoted owing to its role of flocculation, water holding, thickening, adhesion and lubrication. In addition, non-ionic polyacrylamide in other building materials, because of its ultra-high viscosity and its special molecular structure, has good water retention effect.

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  • Preparation of aqueous polyacrylamide solutions for ...

    A process is disclosed for converting polyacrylamide gel into dilute aqueous solutions suitable for use in secondary oil recovery. Polyacrylamide gel is initially converted into a slurry of small gel particles in water from which forms a homogeneous solution concentrate which is then readily diluted to give the final drive fluid without any significant polymer degradation.

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  • Toilet Paper Making Pocess Anionic Polyacrylamide

    Blue native polyacrylamide gel elec- trophoresis (BN-PAGE) is a separation method with a higher resolution than gel filtration or sucrose density ultra- centrifugation that can be used to analyze abundant, stable MPCs from 10 kD to 10 MD.

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  • Synthesis, characterization and evaluation of ...

    New products for rubber additives with promotion price. varieties of products including rubber accelerators,antioxidants,vulcanizing agent, antiscorching agent, rubber …

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  • Blue Native Polyacrylamide Gel Electrophoresis (BN

    (57) Abstract: The method of dehydration and purification gel of polyacrylamide includes freezing, grinding and processing solvent. While remaining solvent from the dehydrated and purified particles are removed by a stream of air or inert gas at a temperature not exceeding 48 o C. expanding the number of solvents used for the dehydration and purification of the gel from impurities.

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  • agarose versus polyacrylamide not all gels bitesize bio ...

    2021-3-1 · Mack and Smith developed a new gel system and called it colloidal dispersion gel (CDG) as via a screen of the viscometer they detected pre-gel aggregates. Consequently, the study indicated that CDG involves a ratio of polymer to cross-linker in the range of 20:1 to 100:1 and allows injection of huge capacity of gels in a cost effective process.

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  • The principle and method of polyacrylamide gel ...

    Preparation of polyacrylamide gel An example performed at MBL Step-by-step procedure; Gather combs, glass plates, spacer (silicone tubing), and binder clips. A comb is used to make wells (lanes) to load samples. Use an appropriate comb depending on the sample size. Example: Use an 8-lane comb for 7 samples and molecular weight markers. ...

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  • Addgene: Protocol - How to Run an Agarose Gel

    Vind de fabrikant Nonionic Polyacrylamide Stollingsmiddel Voor Voedsel Proces van hoge kwaliteit Nonionic Polyacrylamide Stollingsmiddel Voor Voedsel Proces, leverancier en producten voor de laagste prijzen bij Alibaba.com

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  • Ontdek de fabrikant Nonionic Polyacrylamide ...

    2015-1-30 · mation process of copolymer particles was investigated by optical microscope. Keywords: polymer synthesis, aqueous two-phase polymerization, cationic polyacrylamide, dimethylaminoethyl methacry-late methyl chloride, polyethylene glycol 20000 eXPRESS Polymer Letters Vol.4, No.5 (2010) 275–283 Available online at www.expresspolymlett.com

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  • Preparation of cationic polyacrylamide by aqueous two ...

    2011-2-24 · This recipe is sufficient to cast a 30-ml gel. Adjust volumes for the number and size of the gels being poured. 6 15% Separating Gel 3x BN-Gel Buffer (recipe 4) 5.00 mL Acrylamide/Bisacrylamide 5.63 mL Glycerol 70% 4.38 mL APS, 10% in dH 2 O 42 μL

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  • BN-PAGE: Blue Native-Polyacrylamide Gel

    polyacrylamide, flocculant, water treatment Qingdao Oubo. Qingdao Oubo Chemical Co., Ltd. was established in 2011, which is located in the west coast of Qingdao. Our factory specializes in supplying Cationic Polyacrylamide, Anionic Polyacrylamide and Nonionic Polyacrylamide for treatment of industrial waste water and urban sewage.

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  • Native Polyacrylamide Gel Electrophoresis

    Quality Waster Water Treatment Polyacrylamide Flocculant - find quality New products, Other Chemicals & New products from Yitian Bolance Chemicals Co., Ltd. of China Suppliers - 166962565.

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  • Anionic Polyacrylamide Polymer Flocculant MSDS

    Cationically dispersed slurries of alumina pigments, which formed by a process including the combination of one or more alumina pigments, a nonionic wetting agent or nonionic polymer and a cationic interfacial modifier in an aqueous medium in the absence of an acid dispersant. The slurries may be used in a method of manufacturing paper and paperboard products.

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  • Waster Water Treatment Polyacrylamide Flocculant

    An acid composition comprising an acid having a pKA value greater than or equal to about 0.5 and a homopolymer less than about 5.0 having the repeating monomeric structure -CH2CH-(CONH2-)-, and water in an amount sufficient to provide the composition a stable viscosity of about 25 - 20,000 centipoise. The polymer of this invention may be utilized at concentration levels much lower than ...

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