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Get Price1971-1-1 · Bring sample to 1% S D S , 0.01 M sodium p h o s p h a t e , 1% (v/v) 2-mercaptoethanol, and 10% sucrose o r glycerol. 3. H e a t to 100°C for 1 m i n u t e (if necessary). 4a. F o r samples to be stained apply 100 pig of protein in 0.2-0.3 ml on a gel 10 cm long having the desired acrylamide concentration. 4b.
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Get Price2004-11-1 · Pack the column. 1. Wash the gel-filtration medium in a large plastic beaker with 5% ethanol. Let the medium settle and adjust the volume of liquid to yield a gel slurry concentration of 65% to 75%. The XK 16/100, 26/100, and 50/100 columns are 100 cm long and have inner diameters of 16, 26, and 50 mm, …
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Get Price2021-7-8 · Electrophoresis was performed in the presence of SDS on 10% polyacrylamide slabs in 80 mm Tris-glycine buffer, pH 8.6, or on 15% polyacrylamide slabs according to Thomas (23). RESULTS AND DISCUSSION Carbodiimide-catalyzed formation of amide bonds involves as an intermediate step the formation of an …
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