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1984-11-1 · A Pseudomonas sp. which was isolated from phenol-containing soil was immobilized in alginate and polyacrylamide-hydrazide (PAAH) and cultivated in a special airlift fermenter. The immobilized Pseudomonas sp. was able to degrade phenol at initial concentrations up to 2 g/l in less than 2 days, although the free cells did not grow at this concentration.
Get Price2020-7-9 · Recent advances in nanocellulose technology have revealed the potential of crystalline cellulose nanofibers to reinforce materials which are useful for tissue engineering, among o
Get PriceThese temperature and pH values were considered to study the biodegradation of the crystalline cellulose nanofibers in suspension. The encapsulated cellulase preserved its activity for several weeks over that of the free enzyme, which latter considerably decreased and practically showed deactivation after just 10 d.
Get PriceThe spanish carnation (Dianthus caryophyllus L.) is one of the flowers and ornamental plants most requested by the Cuban population. The in vitro propagation of this species with the employment of the analogous of brassinoesteroid Biobras-16, produced in Cuba, …
Get PriceThe esterase mechanism was identified through biochemical assays and polyacrylamide gel electrophoresis. RESULTS: larvae from the Santiago de Cuba strain were susceptible to malathion, pirimiphos, methyl and phenitrothion; there was no variation with the results achieved in a Santiago de Cuba strain in 1997, moderate resistance to phenthion and high resistance to temephos and …
Get Price2010-7-4 · The isoelectric point (pI) was determined by zymograms performed on 5% polyacrylamide gels with a thickness of 1 mm by using a pH range from 3 to 10 (Bio-Rad Ampholine). The sample (20 μl) contained 5–10 mU of laccase. The anode and cathode solutions were 1 M phosphoric acid and 1 M sodium hydroxide, respectively.
Get Price2019-1-1 · A zymogram of cellulolytic activity was performed in a polyacrylamide gel at 12.5% with cellulose included as substrate to be degraded. The enzyme extracts were mixed in a 1:1 ratio with the 2× sample buffer (Tris/HCl 125 mM, 20% (v/v) glycerol, 4% …
Get Price2021-3-12 · One of the most abundant natural polymers on earth, chitin is a fibrous and structural polysaccharide, composed of N -acetyl- d -glucosamine. The biopolymer is the major structural constituent of fungi, arthropods, mollusks, nematodes, and some algae. The biodegradation of chitin is largely manifested by chitinolytic enzyme secreting organisms ...
Get Price1995-3-1 · Hans Leemhuis, Henriëtte J. Rozeboom, Bauke W. Dijkstra, Lubbert Dijkhuizen. The fully conserved Asp residue in conserved sequence region I of the α-amylase family is crucial for the catalytic site architecture and activity. FEBS Letters 2003, 541 (1-3) , …
Get Price2020-7-9 · Recent advances in nanocellulose technology have revealed the potential of crystalline cellulose nanofibers to reinforce materials which are useful for tissue engineering, among o
Get Price2019-7-1 · Graphitic carbon nitride nanosheets (CNS) and TiO 2 nanosheets (TNS) doped with Cu biphenylamine (CuBA) complexes (x-CNS/TNS/CuBA). x-CNS/TNS/CuBA ternary heterostructures were successfully synthesized and applied to pollutants. The SEM, EDS and TEM images clearly reflect the sheetlike morphology of CNS and TNS, this morphology is also maintained in finally sensitized …
Get Price2021-3-12 · One of the most abundant natural polymers on earth, chitin is a fibrous and structural polysaccharide, composed of N-acetyl-d-glucosamine. The biopolymer is the major structural constituent of fungi, arthropods, mollusks, nematodes, and some algae. The biodegradation of chitin is largely manifested by chitinolytic enzyme secreting organisms including bacteria, insects, and plants.
Get PriceINTRODUCTION: resistance to organophosphorus insecticides was diagnosed in Aedes aegypti (Linnaeus, 1762) from Santiago de Cuba in 1997 and some of them are still used up to date; hence the need of ascertaining how the insecticidal resistance has changed in recent times, particularly in 2009. OBJECTIVE: to evaluate the resistance to organophosporus insecticides in larvae from Santiago de Cuba...
Get Price2021-1-1 · Cypermethrin (CYP) is a synthetic pyrethroid broadly used for pest control, however, it is extremely toxic to aquatic organisms. To assess the toxicity of CYP in red claw crayfish Cherax quadricarinatus, transcriptional and proteomic approaches combining two-dimensional polyacrylamide gel electrophoresis and tandem mass spectrometry were used to compare the hepatic expression profiles.
Get Price2011-9-16 · Non-denaturing polyacrylamide gel electrophoresis of the extract, followed by activity staining, revealed one SOD band (estimated M.W. 30.2 kD), at least two CAT bands (estimated M.W. 280 and 350 kD), and at least one POX band (estimated M.W. 45 kD).
Get Price2018-3-1 · 1. Introduction. Humic acids (HA) or more generally, humic substances are produced by the decomposition and biodegradation of organic matter. As one of the most abundant natural substances, humic substances contribute to 60–70% of natural organic materials in soil, and 30–50% of organic constituent in aquatic systems (dos Santos and Oliveira, 2007, Stevenson, 1994), and can be easily …
Get Price1998-12-1 · This listing covers the period July 1, 1997 through to August 31, 1997. Citations are arranged in groups which roughly correspond with the British Myc…
Get Price2019-11-1 · Talc is a magnesium silicate clay mineral and when present in molybdenite concentrate, contributes to the acid-insoluble content. Talc has a laminar structure as shown in Fig. 4.As can be seen, talc is a tri-layered hydrated magnesium silicate with a chemical formula of 3MgO·4SiO 2 ·H 2 O or Mg 3 (Si 2 O 5) 2 (OH) 2 (Xu et al., 2018, Yin et al., 2012), and its crystal structure is similar to ...
Get PriceAnalytical isoelectric focusing polyacrylamide gel electro-phoresis (IEF) (G6rg et al 1980) was performed with a mini-isoelectric focusing cell (Model 111, BioRad, Milan, Italy) by loading 0.2 pLg of protein in 5% polyacrylamide gels containing 20% ampholytes (range 2.5-9.0) (BioRad, Milan, Italy). The anode and cathode solutions were 0.04
Get Price2016-6-10 · Analytical isoelectric focusing polyacrylamide gel electro-phoresis (IEF) (Go¨rg et al 1980) was performed with a mini-isoelectric focusing cell (Model 111, BioRad, Milan, Italy) by loading 0.2 mg of protein in 5% polyacrylamide gels containing 20% ampholytes (range 2.5–9.0) (BioRad, Milan, Italy). The anode and cathode solutions were 0.04
Get Price2019-7-1 · Graphitic carbon nitride nanosheets (CNS) and TiO 2 nanosheets (TNS) doped with Cu biphenylamine (CuBA) complexes (x-CNS/TNS/CuBA). x-CNS/TNS/CuBA ternary heterostructures were successfully synthesized and applied to pollutants. The SEM, EDS and TEM images clearly reflect the sheetlike morphology of CNS and TNS, this morphology is also maintained in finally sensitized …
Get Price2019-1-1 · A zymogram of cellulolytic activity was performed in a polyacrylamide gel at 12.5% with cellulose included as substrate to be degraded. The enzyme extracts were mixed in a 1:1 ratio with the 2× sample buffer (Tris/HCl 125 mM, 20% (v/v) glycerol, 4% …
Get PriceThe spanish carnation (Dianthus caryophyllus L.) is one of the flowers and ornamental plants most requested by the Cuban population. The in vitro propagation of this species with the employment of the analogous of brassinoesteroid Biobras-16, produced in Cuba, allows to obtain a high coefficient of multiplication. However, some of the in vitro culture techniques and some of the bioregulators...
Get Price2011-9-16 · Non-denaturing polyacrylamide gel electrophoresis of the extract, followed by activity staining, revealed one SOD band (estimated M.W. 30.2 kD), at least two CAT bands (estimated M.W. 280 and 350 kD), and at least one POX band (estimated M.W. 45 kD).
Get Price2017-3-8 · Dichomitus squalens is a white-rot fungus that colonizes and grows mainly on softwood and is commonly found in the northern parts of Europe, North America, and Asia. We analyzed the genetic and physiological diversity of eight D. squalens monokaryons derived from a single dikaryon. In addition, an unrelated dikaryon and a newly established dikaryon from two of the studied monokaryons were ...
Get Price1995-3-1 · This article is cited by 111 publications. Chan-Su Rha, Hyoung Geun Kim, Nam-In Baek, Dae-Ok Kim, Cheon-Seok Park. Using Amylosucrase for the Controlled Synthesis of Novel Isoquercitrin Glycosides with Different Glycosidic Linkages.
Get Price1998-12-1 · This listing covers the period July 1, 1997 through to August 31, 1997. Citations are arranged in groups which roughly correspond with the British Myc…
Get PriceAnalytical isoelectric focusing polyacrylamide gel electro-phoresis (IEF) (G6rg et al 1980) was performed with a mini-isoelectric focusing cell (Model 111, BioRad, Milan, Italy) by loading 0.2 pLg of protein in 5% polyacrylamide gels containing 20% ampholytes (range 2.5-9.0) (BioRad, Milan, Italy). The anode and cathode solutions were 0.04
Get PriceThe spanish carnation (Dianthus caryophyllus L.) is one of the flowers and ornamental plants most requested by the Cuban population. The in vitro propagation of this species with the employment of the analogous of brassinoesteroid Biobras-16, produced in Cuba, allows to obtain a high coefficient of multiplication. However, some of the in vitro culture techniques and some of the bioregulators...
Get PriceENVIRONMENTAL MICROBIOLOGY AND BIODEGRADATION - Novel Alkylsulfatases Required for Biodegradation of the Branched Primary Alkyl Sulfate Surfactant 2-Butyloctyl Sulfate Ellis, Andrew J. Hales, Stephen G. Ur-Rehman, Naheed G.A. White, Graham F. | 2002
Get Price2012-11-28 · Polyacrylamide gel electrophoresis (PAGE) SteLL was evaluated using PAGE in the presence of sodium dodecyl sulfate (SDS-PAGE) and β-mercaptoethanol on a 15% (w/v) gel according to Laemmli . Polypeptide bands and molecular mass standards (bovine serum albumin, 66 000 Da, ovalbumin, 45 000 Da, glyceraldehyde-3-phosphate dehydrogenase, 36 000 Da ...
Get Price1995-3-1 · This article is cited by 111 publications. Chan-Su Rha, Hyoung Geun Kim, Nam-In Baek, Dae-Ok Kim, Cheon-Seok Park. Using Amylosucrase for the Controlled Synthesis of Novel Isoquercitrin Glycosides with Different Glycosidic Linkages.
Get PriceA review of 133 studies for fuel hydrocarbons has yielded first-order biodegradation coefficients up to 0.445 day−1 under aerobic conditions and up to 0.522−1 under anaerobic conditions in 90% of the cases.
Get PriceOpslaan van 100 µL van raw lysate p.a. natrium dodecyl sulfaat-polyacrylamide gel elektroforese (SDS-pagina) aan het einde van de zuivering. Na de laatste dooi, de pH van de ontdooide lysate tot 9.0 met behulp van de 1 M NaOH te regelen.
Get PriceIsolation and Characterization of Polyacrylamide-Degrading Bacteria from Dewatered Sludge. Previous Article in Journal. ... A Low-Cost Wheat Bran Medium for Biodegradation of the Benzidine-Based Carcinogenic Dye Trypan Blue Using a Microbial Consortium. 25th Anniversary Journals. Information.
Get Price2016-4-18 · The obtained PCR products underwent electrophoresis in a concentration gradient in a DCode universal mutation detection system (Bio-Rad, Hercules, CA, USA). The amplicons with a GC tail were separated in 8% polyacrylamide gel (acrylamide-to-bisacrylamide ratio, 39.5:1) containing 30% to 60% of the denaturation factor (urea).
Get PriceBiodegradation of cellulose is mediated Microbial growth on canvas paintings results in deterioration of by the cellulolytic enzyme pool secreted by microorganisms, these artworks and is observed as aesthetic (e.g. pigment produc- especially fungi, giving rise to glucose production as well as other tion), chemical (e.g. enzymes and organic ...
Get PriceThis is the 34 th annual review of the application of atomic spectrometry to the chemical analysis of environmental samples. This Update refers to papers published approximately between August 2017 and June 2018 and continues the series of Atomic Spectrometry Updates (ASUs) in Environmental Analysis 1 that should be read in conjunction with other related ASUs in the series, namely: clinical ...
Get Price2019-11-26 · Desalination and Water Treatment desatercom doi10004/dt201822284 Contents Volume 104 104 (2018) v–vi eruar Experimental study on lens type solar still with single slope and single basin
Get Price2019-9-9 · Biodegradation of endocrine disruptors in urban wastewater using Pleurotus ostreatus bioreactor. New Biotechnology, Vol. 43. ... Microwave Enhanced Synthesis of Chitosan-Graft-Polyacrylamide Molecular Imprinting Polymer for Selective Removal of 17β-Estradiol at Trace Concentration. Asian Journal of Biochemistry, Vol. 6, No. 1.
Get Price2008-6-17 · The Rhizobium sp. DehL was produced by heterologous expression of the cloned gene in Escherichia coli. DehL enzyme was purified to homogeneity and characterized. The molecular weights were estimated to be 61 and 31 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), respectively, suggesting that the enzyme is a dimer.
Get Price2015-1-12 · The search is on, therefore, for environmentally compatible alternatives, such as recovery of raw materials, CO 2-neutral thermal utilisation or – in certain circumstances – biodegradation. Another interesting option may be that of construction materials which are developed from renewable resources; in this case, natural fibres are embedded ...
Get PriceOpslaan van 100 µL van raw lysate p.a. natrium dodecyl sulfaat-polyacrylamide gel elektroforese (SDS-pagina) aan het einde van de zuivering. Na de laatste dooi, de pH van de ontdooide lysate tot 9.0 met behulp van de 1 M NaOH te regelen.
Get PriceElectrophoretic Elution of Macromolecules from Polyacrylamide or Agarose Gels Atmeh, Ragheb F. Abu-Harfiel, Nizar M. El-Sukhon, Saeb N. Abo-Shehada, Mahmoud N. | 1991 digital version
Get Price2019-9-9 · Biodegradation of endocrine disruptors in urban wastewater using Pleurotus ostreatus bioreactor. New Biotechnology, Vol. 43. ... Microwave Enhanced Synthesis of Chitosan-Graft-Polyacrylamide Molecular Imprinting Polymer for Selective Removal of 17β-Estradiol at Trace Concentration. Asian Journal of Biochemistry, Vol. 6, No. 1.
Get Price2012-11-28 · Polyacrylamide gel electrophoresis (PAGE) SteLL was evaluated using PAGE in the presence of sodium dodecyl sulfate (SDS‐PAGE) and β‐mercaptoethanol on a 15% (w/v) gel according to Laemmli . Polypeptide bands and molecular mass standards (bovine serum albumin, 66 000 Da, ovalbumin, 45 000 Da, glyceraldehyde‐3‐phosphate dehydrogenase, 36 ...
Get PriceENVIRONMENTAL MICROBIOLOGY AND BIODEGRADATION - Novel Alkylsulfatases Required for Biodegradation of the Branched Primary Alkyl Sulfate Surfactant 2-Butyloctyl Sulfate Ellis, Andrew J. Hales, Stephen G. Ur-Rehman, Naheed G.A. White, Graham F. | 2002
Get Price2020-7-14 · Cell 12:1097 1108 Jeffries TW (1990) Biodegradation of lignin carbohydr ate complexes. Biodegradation 1 :163 176 Jung JH, Xiong Y, Kim JY, Fouad W, Vermerris W, Gallo M, Altpeter F (2011) RNA Interference Suppresses Lignin Biosynthetic Genes Caffeic Acid 3 O methyltransferase ( COMT ) and/or 4 Coumarate CoA Ligase ( 4CL ) in Sugarcane.
Get Price2018-7-4 · Downy mildew in cucumber plants, caused by Pseudoperonospora cubensis, was reduced by foliar spraying of antitranspirants film Kaolin, Nu-Film, Bio-Film, Folicote and Polyacrylamide Anti-Stress 550. In pots experiments, antitranpirants were proved to be effective in reducing disease incidence, severity and pathogen sporulation whatever prior or post inoculated.
Get Price2016-4-27 · Microbial activity has an important impact on the maintenance of cultural heritage materials, owing to the key role of microorganisms in many deterioration processes. In order to minimize such deleterious effects, there is a need to fine-tune methods that detect and characterize microorganisms. Trends in microbiology indicate that this need can be met by incorporating modern techniques.
Get Price2020-10-22 · Foodomics is an emerging research field in food science that applies advanced omics technologies to assess relevant aspects related to food and nutrition, with the ultimate goal to improve human health and well-being. Many studies have already shown the tremendous potential of this approach to boost food science research regarding food authentication and traceability, safety issues, …
Get Price2020-7-9 · Recent advances in nanocellulose technology have revealed the potential of crystalline cellulose nanofibers to reinforce materials which are useful for tissue engineering, among o
Get Price2019-7-1 · Graphitic carbon nitride nanosheets (CNS) and TiO 2 nanosheets (TNS) doped with Cu biphenylamine (CuBA) complexes (x-CNS/TNS/CuBA). x-CNS/TNS/CuBA ternary heterostructures were successfully synthesized and applied to pollutants. The SEM, EDS and TEM images clearly reflect the sheetlike morphology of CNS and TNS, this morphology is also maintained in finally sensitized …
Get Price2021-3-12 · One of the most abundant natural polymers on earth, chitin is a fibrous and structural polysaccharide, composed of N-acetyl-d-glucosamine. The biopolymer is the major structural constituent of fungi, arthropods, mollusks, nematodes, and some algae. The biodegradation of chitin is largely manifested by chitinolytic enzyme secreting organisms including bacteria, insects, and plants.
Get PriceINTRODUCTION: resistance to organophosphorus insecticides was diagnosed in Aedes aegypti (Linnaeus, 1762) from Santiago de Cuba in 1997 and some of them are still used up to date; hence the need of ascertaining how the insecticidal resistance has changed in recent times, particularly in 2009. OBJECTIVE: to evaluate the resistance to organophosporus insecticides in larvae from Santiago de Cuba...
Get Price2021-1-1 · Cypermethrin (CYP) is a synthetic pyrethroid broadly used for pest control, however, it is extremely toxic to aquatic organisms. To assess the toxicity of CYP in red claw crayfish Cherax quadricarinatus, transcriptional and proteomic approaches combining two-dimensional polyacrylamide gel electrophoresis and tandem mass spectrometry were used to compare the hepatic expression profiles.
Get Price2011-9-16 · Non-denaturing polyacrylamide gel electrophoresis of the extract, followed by activity staining, revealed one SOD band (estimated M.W. 30.2 kD), at least two CAT bands (estimated M.W. 280 and 350 kD), and at least one POX band (estimated M.W. 45 kD).
Get Price2018-3-1 · 1. Introduction. Humic acids (HA) or more generally, humic substances are produced by the decomposition and biodegradation of organic matter. As one of the most abundant natural substances, humic substances contribute to 60–70% of natural organic materials in soil, and 30–50% of organic constituent in aquatic systems (dos Santos and Oliveira, 2007, Stevenson, 1994), and can be easily …
Get Price1998-12-1 · This listing covers the period July 1, 1997 through to August 31, 1997. Citations are arranged in groups which roughly correspond with the British Myc…
Get Price2019-11-1 · Talc is a magnesium silicate clay mineral and when present in molybdenite concentrate, contributes to the acid-insoluble content. Talc has a laminar structure as shown in Fig. 4.As can be seen, talc is a tri-layered hydrated magnesium silicate with a chemical formula of 3MgO·4SiO 2 ·H 2 O or Mg 3 (Si 2 O 5) 2 (OH) 2 (Xu et al., 2018, Yin et al., 2012), and its crystal structure is similar to ...
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